CAJ | 학술논문

利用 pET-28a(＋)质粒将来源于耐辐射异常球菌(Deinococcus radiodurans)R1的 irrE 基因在大肠杆菌(Escherichia coli)BL21(DE3)中进行异源表达，在IPTG终浓度2,mmol/L、诱导温度37,℃、诱导培养6,h条件下进行了蛋白诱导。进一步考察 IrrE 异源表达对大肠杆菌生长性能和耐受能力的影响，结果表明：IrrE 的表达能够提高大肠杆菌正常条件下的生长速率和最终生物量；同时，能够不同程度提高大肠杆菌在压力冲击下的存活能力，其中，高渗条件下存活能力的提高最为明显。而重组菌株在压力冲击下的生长速率和最终生物量均明显高于对照菌株。这说明 IrrE在提高大肠杆菌的压力耐受性方面表现出良好的效果。
이용 pET-28a(＋)질립장래원우내복사이상구균(Deinococcus radiodurans)R1적 irrE 기인재대장간균(Escherichia coli)BL21(DE3)중진행이원표체，재IPTG종농도2,mmol/L、유도온도37,℃、유도배양6,h조건하진행료단백유도。진일보고찰 IrrE 이원표체대대장간균생장성능화내수능력적영향，결과표명：IrrE 적표체능구제고대장간균정상조건하적생장속솔화최종생물량；동시，능구불동정도제고대장간균재압력충격하적존활능력，기중，고삼조건하존활능력적제고최위명현。이중조균주재압력충격하적생장속솔화최종생물량균명현고우대조균주。저설명 IrrE재제고대장간균적압력내수성방면표현출량호적효과。
The heterologous expression of irrE,from Deinococcus radiodurans R1,in E. coli BL21(DE3)was carried out using the vector pET-28a(+). The optimal protein expression conditions of IrrE were as follows.The final concentration of IPTG was 2,mmol/L,and the induction temperature and time were 37,℃and 6,h,respectively. The influence of IrrE on cell growth and tolerance was further investigated. The results indicated that IrrE could enhance the growth rate and produce more final biomass of Escherichia coli under normal conditions,and improve the survival ability of the cell under stress shock to different extent,especially the tolerance to osmotic shock. The growth rate was higher and significantly more final biomasses of the recombinant strain containing pET-28a(+)-irrE under stress were found than in the control strain(harboring the empty vector pET-28a(+)). It can be concluded that the expression of IrrE could enhance the tolerance of E. coli to stress.