南方水产科学
南方水產科學
남방수산과학
SOUTH CHINA FISHERIES SCIENCE
2015年
2期
12-19
,共8页
蔡星媛%张秀梅%田璐%张沛东
蔡星媛%張秀梅%田璐%張沛東
채성원%장수매%전로%장패동
盐度%魁蚶%鳃%血淋巴渗透压%Na+/K+-ATP酶
鹽度%魁蚶%鰓%血淋巴滲透壓%Na+/K+-ATP酶
염도%괴감%새%혈림파삼투압%Na+/K+-ATP매
salinity%Anadara broughtonii%gill%hemolymph osmolality%Na+ /K+-ATPase activity
文章研究了盐度胁迫下魁蚶( Anadara broughtonii)稚贝(壳长25~35 mm)血淋巴渗透压和鳃Na+/K+-ATP酶活力的变化。结果显示,突变胁迫下,盐度20、25、35、40组的稚贝血淋巴渗透压均在48 h内显著降低或波动升高后趋于稳定,盐度15组则需96 h完成波动调整。以日变幅5渐变至高盐40过程中,稚贝血淋巴渗透压在48 h内趋于稳定;低盐15组仅需72 h完成渗透压调节,调整时间显著小于突变组。盐度突降胁迫下,各处理组稚贝鳃Na+/K+-ATP酶活力12 h后显著升高,24 h分别达到最大值,随后快速回落并稳定在高于对照组水平;盐度骤升胁迫下,鳃ATP酶活力波动降低,在12~24 h达到最小值后稳定在低于对照组水平。低盐(15)和高盐(40)渐变组的鳃ATP酶活力变幅均小于突变组。盐度胁迫下鳃Na+/K+-ATP酶活力的响应方式不同,即低盐胁迫时活力增强,高盐胁迫时活力降低。采用盐度渐变的驯化处理更有利于魁蚶稚贝应对盐度胁迫。
文章研究瞭鹽度脅迫下魁蚶( Anadara broughtonii)稚貝(殼長25~35 mm)血淋巴滲透壓和鰓Na+/K+-ATP酶活力的變化。結果顯示,突變脅迫下,鹽度20、25、35、40組的稚貝血淋巴滲透壓均在48 h內顯著降低或波動升高後趨于穩定,鹽度15組則需96 h完成波動調整。以日變幅5漸變至高鹽40過程中,稚貝血淋巴滲透壓在48 h內趨于穩定;低鹽15組僅需72 h完成滲透壓調節,調整時間顯著小于突變組。鹽度突降脅迫下,各處理組稚貝鰓Na+/K+-ATP酶活力12 h後顯著升高,24 h分彆達到最大值,隨後快速迴落併穩定在高于對照組水平;鹽度驟升脅迫下,鰓ATP酶活力波動降低,在12~24 h達到最小值後穩定在低于對照組水平。低鹽(15)和高鹽(40)漸變組的鰓ATP酶活力變幅均小于突變組。鹽度脅迫下鰓Na+/K+-ATP酶活力的響應方式不同,即低鹽脅迫時活力增彊,高鹽脅迫時活力降低。採用鹽度漸變的馴化處理更有利于魁蚶稚貝應對鹽度脅迫。
문장연구료염도협박하괴감( Anadara broughtonii)치패(각장25~35 mm)혈림파삼투압화새Na+/K+-ATP매활력적변화。결과현시,돌변협박하,염도20、25、35、40조적치패혈림파삼투압균재48 h내현저강저혹파동승고후추우은정,염도15조칙수96 h완성파동조정。이일변폭5점변지고염40과정중,치패혈림파삼투압재48 h내추우은정;저염15조부수72 h완성삼투압조절,조정시간현저소우돌변조。염도돌강협박하,각처리조치패새Na+/K+-ATP매활력12 h후현저승고,24 h분별체도최대치,수후쾌속회락병은정재고우대조조수평;염도취승협박하,새ATP매활력파동강저,재12~24 h체도최소치후은정재저우대조조수평。저염(15)화고염(40)점변조적새ATP매활력변폭균소우돌변조。염도협박하새Na+/K+-ATP매활력적향응방식불동,즉저염협박시활력증강,고염협박시활력강저。채용염도점변적순화처리경유리우괴감치패응대염도협박。
The study investigated the variations of hemolymph osmolality and gill Na+/K+-ATPase activity in juvenile ark shell( Anada-ra broughtonii)( shell length 25~35 mm)under different salinity stresses. The results show that the hemolymph osmolality under ab-rupt stress at salinities of 20,25,35 and 40 decreased significantly or fluctuated ascendingly then became stable in 48 h. At salinity of 15,the juveniles needed 96 h to complete the adjustment. At a changing salinity rate of 5·d-1 ,the hemolymph osmolality at salini-ty of 40 tended to be stable in 48 h but in 72 h at salinity of 15,which was faster than at salinity of 15. After an abrupt decrease in sa-linity,the Na+/K+-ATPase activity of gills increased significantly in 12 h and each treatment reached its peak in 24 h. Then the activi-ty quickly dropped and returned to a steady state higher than the control,and with an abrupt increase of salinity,it fluctuated de-scendingly and reached the bottom in 12~24 h lower than the control. The activity changed much less at salinity of 15 and 40 than at sudden-change salinity. In conclusion,the response patterns of Na+/K+-ATPase activity of gills under different salinity stresses were different,i. e. ,the activity increased under low salinity stress but decreased under high salinity stress. It is suggested that gradual sa-linity change would be helpful for A. broughtonii to adapt to salinity stress.