中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2015年
4期
382-386
,共5页
杨德刚%李建军%杨明亮%杜良杰%胡安明%顾蕊%陈亮%褚宏宇%张宪娣%吴海丰
楊德剛%李建軍%楊明亮%杜良傑%鬍安明%顧蕊%陳亮%褚宏宇%張憲娣%吳海豐
양덕강%리건군%양명량%두량걸%호안명%고예%진량%저굉우%장헌제%오해봉
脊髓损伤%自噬%Beclin-1%Bcl-2%脊髓切开术
脊髓損傷%自噬%Beclin-1%Bcl-2%脊髓切開術
척수손상%자서%Beclin-1%Bcl-2%척수절개술
spinal cord injury%autophagy%Beclin-1%Bcl-2%myelotomy
目的：观察脊髓切开术干预后大鼠脊髓损伤区自噬活性的变化。方法54只成年雌性Sprague-Dawley大鼠随机分为假手术组(n=18)、挫伤组(n=18)和手术组(n=18)。用NYU打击器制备T10脊髓挫伤模型。手术组在造模后24 h行脊髓切开术。在伤后第1、7、14天，采用开放场地试验评估大鼠运动功能；RT-PCR检测Beclin-1和Bcl-2的mRNA表达；伤后3 d开始电镜观察自噬体。结果损伤后7 d、14 d，手术组大鼠BBB评分高于挫伤组(P<0.05)，Beclin-1 mRNA水平低于挫伤组(P<0.05)；伤后3 d、7 d，Bcl-2 mRNA水平高于挫伤组(P<0.05)。伤后7 d、14 d，大鼠脊髓损伤区的Beclin-1 mRNA水平与行为学评分负相关(P<0.01)。脊髓挫伤后自噬体形成增多，手术组自噬体形成减少。结论脊髓切开术能改善创伤性脊髓损伤大鼠的运动功能，可能与抑制自噬、上调Bcl-2 mRNA表达有关。
目的：觀察脊髓切開術榦預後大鼠脊髓損傷區自噬活性的變化。方法54隻成年雌性Sprague-Dawley大鼠隨機分為假手術組(n=18)、挫傷組(n=18)和手術組(n=18)。用NYU打擊器製備T10脊髓挫傷模型。手術組在造模後24 h行脊髓切開術。在傷後第1、7、14天，採用開放場地試驗評估大鼠運動功能；RT-PCR檢測Beclin-1和Bcl-2的mRNA錶達；傷後3 d開始電鏡觀察自噬體。結果損傷後7 d、14 d，手術組大鼠BBB評分高于挫傷組(P<0.05)，Beclin-1 mRNA水平低于挫傷組(P<0.05)；傷後3 d、7 d，Bcl-2 mRNA水平高于挫傷組(P<0.05)。傷後7 d、14 d，大鼠脊髓損傷區的Beclin-1 mRNA水平與行為學評分負相關(P<0.01)。脊髓挫傷後自噬體形成增多，手術組自噬體形成減少。結論脊髓切開術能改善創傷性脊髓損傷大鼠的運動功能，可能與抑製自噬、上調Bcl-2 mRNA錶達有關。
목적：관찰척수절개술간예후대서척수손상구자서활성적변화。방법54지성년자성Sprague-Dawley대서수궤분위가수술조(n=18)、좌상조(n=18)화수술조(n=18)。용NYU타격기제비T10척수좌상모형。수술조재조모후24 h행척수절개술。재상후제1、7、14천，채용개방장지시험평고대서운동공능；RT-PCR검측Beclin-1화Bcl-2적mRNA표체；상후3 d개시전경관찰자서체。결과손상후7 d、14 d，수술조대서BBB평분고우좌상조(P<0.05)，Beclin-1 mRNA수평저우좌상조(P<0.05)；상후3 d、7 d，Bcl-2 mRNA수평고우좌상조(P<0.05)。상후7 d、14 d，대서척수손상구적Beclin-1 mRNA수평여행위학평분부상관(P<0.01)。척수좌상후자서체형성증다，수술조자서체형성감소。결론척수절개술능개선창상성척수손상대서적운동공능，가능여억제자서、상조Bcl-2 mRNA표체유관。
Objective To observe the effects of myelotomy on autophagy activation after traumatic spinal cord injury (SCI) in rats. Methods 54 adult female Sprague-Dawley rats were randomly assigned to sham-operated group (SG, n=18), contusion group (CG, n=18) or myelotomy group (MTG, n=18). The T10 SCI model in rats was induced with a New York University (NYU) impactor and myelotomy was performed 24 hours after SCI. They were evaluated with the BBB score 1, 7, 14 days after injury. The expression of mRNA of Beclin-1 and Bcl-2 were detected with real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The formation of autophagosome was investigated under electronic microscope (EM) 3 days after injury. Results BBB score was more in the MTG than in the CG 7 and 14 days after injury (P<0.05), while the expression of Beclin-1 mRNA was less (P<0.05). The expression of Bcl-2 mRNA was more in the MTG than in the CG 3 and 7 days after injury (P<0.05). The expression of Beclin-1 mRNA was negatively correlated with BBB scores (P<0.05). The formation of autophagosome was less in the MTG than in the CG. Conclusion Myelotomy can improve the recovery of motor function in rats after acute traumatic SCI, which may associate with neuroprotection mediated by inhibition of autophagy through the Bcl-2 signaling pathway.
