CAJ | 학술논문

目的：观察8周有氧运动对慢性心力衰竭大鼠骨骼肌糖原含量和运动耐力的影响和可能机制。方法30只Sprague-Dawley大鼠分为心力衰竭安静组(H组，n=10)、心力衰竭运动组(HT组，n=10)和假手术组(S组，n=10)，采用冠状动脉结扎术复制心力衰竭模型。HE组进行8周跑台训练。干预后用蒽酮法测定骨骼肌糖原含量，递增负荷力竭运动试验测定力竭时间，放射性同位素法测定葡萄糖摄取率、骨骼肌糖原合酶(GS)和糖原磷酸化酶(GP)活性，Western Blotting法测定总GS、磷酸化GS (p-GS)、总GP以及磷酸化GP(p-GP)蛋白表达；对糖原含量和力竭时间进行相关分析。结果与S组大鼠比较，H组骨骼肌糖原含量、力竭时间、胰岛素介导的葡萄糖摄取率、GS活性和p-GP蛋白表达量降低(P<0.05)，血清胰岛素、GP活性和p-GS蛋白表达量升高(P<0.05)；与H组比较，HT组骨骼肌糖原含量、力竭时间、胰岛素介导的葡萄糖摄取率、GS活性和p-GP蛋白表达量增加(P<0.05)，血清胰岛素、GP活性和p-GS蛋白表达量降低(P<0.05)。糖原含量和力竭时间显著正相关(P<0.05)。结论长期有氧运动能提高慢性心力衰竭大鼠骨骼肌糖原含量和运动耐力，可能与胰岛素敏感性改善，葡萄糖摄取增加，糖原合成增多、分解减少有关。
목적：관찰8주유양운동대만성심력쇠갈대서골격기당원함량화운동내력적영향화가능궤제。방법30지Sprague-Dawley대서분위심력쇠갈안정조(H조，n=10)、심력쇠갈운동조(HT조，n=10)화가수술조(S조，n=10)，채용관상동맥결찰술복제심력쇠갈모형。HE조진행8주포태훈련。간예후용은동법측정골격기당원함량，체증부하력갈운동시험측정력갈시간，방사성동위소법측정포도당섭취솔、골격기당원합매(GS)화당원린산화매(GP)활성，Western Blotting법측정총GS、린산화GS (p-GS)、총GP이급린산화GP(p-GP)단백표체；대당원함량화력갈시간진행상관분석。결과여S조대서비교，H조골격기당원함량、력갈시간、이도소개도적포도당섭취솔、GS활성화p-GP단백표체량강저(P<0.05)，혈청이도소、GP활성화p-GS단백표체량승고(P<0.05)；여H조비교，HT조골격기당원함량、력갈시간、이도소개도적포도당섭취솔、GS활성화p-GP단백표체량증가(P<0.05)，혈청이도소、GP활성화p-GS단백표체량강저(P<0.05)。당원함량화력갈시간현저정상관(P<0.05)。결론장기유양운동능제고만성심력쇠갈대서골격기당원함량화운동내력，가능여이도소민감성개선，포도당섭취증가，당원합성증다、분해감소유관。
Objective To observe the effects of 8-week aerobic exercise on skeletal muscle glycogen content and exercise endurance and investigate the possible mechanism in rats with chronic heart failure. Methods 30 Sprague-Dawley rats were submitted to heart failure sed-entary (H) group, heart failure traning (HT) group and sham operation (S) group. The heart failure model was established with coronary ar-tery ligation. HT group performed an 8-week treadmill running. The skeletal muscle glycogen content was determined with anthracenone, exercise endurance with exhaust duration of graded exhausted exercise test, glucose uptake rate, activity of glycogen synthase (GS) and gly-cogen phosphorylase (GP) with radicisotope, protein expression of total GS, phospho-GS (p-GS), total GP and phospho-GP (p-GP) with Western Blotting. Correlation analysis was conducted between glycogen content and exhaust duration. Results The skeletal muscle glyco-gen, exhaust duration, glucose uptake rate, GS activity and p-GP protein reduced (P<0.05), while serum insulin, GP activity and p-GS pro-tein raised (P<0.05) in the H group compared with those in the S group. The skeletal muscle glycogen, exhaust duration, glucose uptake rate, GS activity and p-GP protein increased (P<0.05), while serum insulin, GP activity and p-GS protein decreased (P<0.05) in the HT group compared with those in the H group. There was positive correlation between glycogen content and exhaust duration (P<0.05). Conclu-sion Prolonged aerobic exercise enhanced skeletal muscle glycogen content and exercise endurance in rats with chronic heart failure, which may be related to the improvement of insulin sensitivity, muscle glucose uptake, increase of glycogen synthesis and decrease of glycogen breakdown.