CAJ | 학술논문

目的：观察夹脊电针和神经松动术对兔坐骨神经损伤后轴突再生和血清脑源性神经营养因子(BDNF)、睫状神经营养因子(CNTF)含量的影响。方法30只成年雄性兔分为模型组(n=6)、假手术组(n=6)、神经松动术组(n=6)、夹脊电针组(n=6)、夹脊电针结合神经松动术组(n=6)。钳夹法复制坐骨神经损伤模型。模型组、假手术组不做任何干预，神经松动术组行神经松动术治疗，夹脊电针组进行夹脊电针治疗，夹电针结合神经松动术组进行夹脊电针和神经松动术治疗。治疗4周后，HE染色观察轴突生长情况，ELISA法检测血清BDNF、CNTF含量。结果神经松动术组、夹脊电针组、夹脊电针结合神经松动术组轴突生长情况均优于模型组，夹脊电针结合神经松动术组优于神经松动术组和夹脊电针组；神经松动术组、夹脊电针组、夹脊电针结合神经松动术组血清BDNF、CNTF含量均高于模型组(P<0.05)，夹脊电针结合神经松动术组优于神经松动术组和夹脊电针组(P<0.05)。结论神经松动术、夹脊电针均可促进兔损伤坐骨神经的轴突再生，可能与提高血清中CNTF、BDNF水平有关；两者结合效果更佳。
목적：관찰협척전침화신경송동술대토좌골신경손상후축돌재생화혈청뇌원성신경영양인자(BDNF)、첩상신경영양인자(CNTF)함량적영향。방법30지성년웅성토분위모형조(n=6)、가수술조(n=6)、신경송동술조(n=6)、협척전침조(n=6)、협척전침결합신경송동술조(n=6)。겸협법복제좌골신경손상모형。모형조、가수술조불주임하간예，신경송동술조행신경송동술치료，협척전침조진행협척전침치료，협전침결합신경송동술조진행협척전침화신경송동술치료。치료4주후，HE염색관찰축돌생장정황，ELISA법검측혈청BDNF、CNTF함량。결과신경송동술조、협척전침조、협척전침결합신경송동술조축돌생장정황균우우모형조，협척전침결합신경송동술조우우신경송동술조화협척전침조；신경송동술조、협척전침조、협척전침결합신경송동술조혈청BDNF、CNTF함량균고우모형조(P<0.05)，협척전침결합신경송동술조우우신경송동술조화협척전침조(P<0.05)。결론신경송동술、협척전침균가촉진토손상좌골신경적축돌재생，가능여제고혈청중CNTF、BDNF수평유관；량자결합효과경가。
Objective To observe the effects of Jiaji electroacupuncture and neurodynamic mobilization technique on axon regeneration and content of brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) in rabbits with sciatic nerve injury. Meth-ods 30 male adult rabbits were assigned to sham (A, n=6), model (B, n=6), neurodynamic mobilization (C, n=6), Jiaji electroacupuncture (D, n=6), and Jiaji electroacupuncture combine with neurodynamic mobilization (E, n=6) groups. The group C was treated with neurodynam-ic mobilization, the group D with Jiaji electroacupuncture, and the group D with both 3 days after modeling of clamping at sciatic nerve, while the groups A and B with no treatment. The axon regeneration was observed with HE staining, and the content of BDNF and CNTF in serum was measured with ELISA 4 weeks after treatment. Results The axons regeneration was observed better in the groups C, D and E than in the group B. The content of BDNF and CNTF was more in the groups C, D and E than in the group B (P<0.05). Conclusion Both Ji-aji electroacupuncture and neurodynamic mobilization can improve axon regeneration with synergistic action, which may associate with the increase of BDNF and CNTF in serum.