CAJ | 학술논문

目的：探讨丁苯酞对l-甲基-4-苯基-吡啶离子(MPP+)诱导的SH-SY5Y细胞凋亡的影响。方法 SH-SY5Y细胞加入1μmol/L、10μmol/L、20μmol/L丁苯酞培养2 h，加入MPP+培养24 h。台盼蓝染色检测SH-SY5Y细胞存活率；罗丹明123染色检测线粒体膜电位的变化；ELISA检测胞浆P53的含量；Western blotting检测胞浆细胞色素C (CytC)、P53的表达。结果随丁苯酞剂量提高，SH-SY5Y细胞存活率提高(P<0.05)，线粒体膜电位降低(P<0.05)，P53和CytC蛋白表达下降(P<0.05)。结论丁苯酞能够抵抗MPP+诱导的SH-SY5Y细胞凋亡，其机制可能是通过抑制P53、CytC的表达，增加细胞线粒体膜稳定性。
목적：탐토정분태대l-갑기-4-분기-필정리자(MPP+)유도적SH-SY5Y세포조망적영향。방법 SH-SY5Y세포가입1μmol/L、10μmol/L、20μmol/L정분태배양2 h，가입MPP+배양24 h。태반람염색검측SH-SY5Y세포존활솔；라단명123염색검측선립체막전위적변화；ELISA검측포장P53적함량；Western blotting검측포장세포색소C (CytC)、P53적표체。결과수정분태제량제고，SH-SY5Y세포존활솔제고(P<0.05)，선립체막전위강저(P<0.05)，P53화CytC단백표체하강(P<0.05)。결론정분태능구저항MPP+유도적SH-SY5Y세포조망，기궤제가능시통과억제P53、CytC적표체，증가세포선립체막은정성。
Objective To explore the effects of butylphthalide on the apoptosis of SH-SY5Y cells induced by the 1-methyl-4-phenylpyri-dinium iodide (MPP+). Methods SH-SY5Y cells were cultured with butylphthalide in the dosage of 1 μmol/L, 10 μmol/L and 20 μmol/L for 2 hours, and with MPP+for 24 hours. The viability of cells was measured with trypan blue. The mitochondrial membrane potential was de-tected with Rhodamine 123. The content of P53 protein was detected with ELISA. The expression of P53 and cytochrome C (CytC) were de-tected with Western blotting. Results The viability of SH-SY5Y cells increased with the dosage of butylphthalide (P<0.05), while the mito-chondrial membrane potential and the expression of P53 and CytC decreased (P<0.05). Conclusion Butylphthalide can prevents the SH-SY5Y cells from the injury induced by MPP+, which may associate with the inhibition of apoptosis through the expression of P53 and CytC.