CAJ | 학술논문

目的：研究大鼠重度脊髓损伤后损伤中心不同白质束的病理变化。方法21只成年雌性Wistar大鼠，分为正常对照组(n=3)、假手术组(n=3)和脊髓损伤组(n=15)。损伤组使用NYU脊髓损伤打击器10 g×50 mm制备大鼠T7-8节段重度脊髓损伤模型。损伤后1 d、3 d、7 d、14 d和28 d通过快蓝染色(LFB染色)、NF200免疫组织化学染色检测损伤脊髓的病理改变，旷场Basso-Beat-tie-Bresnahan(BBB)法评价大鼠行为学变化。结果大鼠后肢运动功能在损伤后3 d开始自发性恢复，在14~28 d达到平台期。LFB染色显示，损伤中心腹侧束白质和外侧束白质有残留，残留白质面积百分比在损伤后3 d降至最低，7~28 d为平台期。免疫组化染色显示，损伤中心不同白质束中NF200阳性轴突数目在伤后明显减少。结论脊髓损伤后，腹侧白质残留对大鼠后肢运动功能恢复起关键作用，伤后3d为脊髓损伤急性期保护残留白质的关键时间窗。
목적：연구대서중도척수손상후손상중심불동백질속적병리변화。방법21지성년자성Wistar대서，분위정상대조조(n=3)、가수술조(n=3)화척수손상조(n=15)。손상조사용NYU척수손상타격기10 g×50 mm제비대서T7-8절단중도척수손상모형。손상후1 d、3 d、7 d、14 d화28 d통과쾌람염색(LFB염색)、NF200면역조직화학염색검측손상척수적병리개변，광장Basso-Beat-tie-Bresnahan(BBB)법평개대서행위학변화。결과대서후지운동공능재손상후3 d개시자발성회복，재14~28 d체도평태기。LFB염색현시，손상중심복측속백질화외측속백질유잔류，잔류백질면적백분비재손상후3 d강지최저，7~28 d위평태기。면역조화염색현시，손상중심불동백질속중NF200양성축돌수목재상후명현감소。결론척수손상후，복측백질잔류대대서후지운동공능회복기관건작용，상후3d위척수손상급성기보호잔류백질적관건시간창。
Objective To explore pathological characteristics of different white matter tracts at core of the spinal cord injury (SCI) in adult rats. Methods 21 adult female Wistar rats were divided into normal group (n=3), sham group (n=3) and lesion group (n=15). The rats of the lesion group were severely injured at T7-8 using the NYU impactor of 10 g×50 mm. The pathology of spinal cord injury was detected using Luxol fast blue (LFB) staining and NF200 immunohistochemistry staining 1 day, 3 days, 7 days, 14 days and 28 days after injury, while the hindlimbs behavior of rats was rated with Basso-Beattie-Bresnahan (BBB) scores of open-field. Results The BBB scores recov-ered 3 days after injury and reached a platform from 14 to 28 days after injury. LFB showed that there were spared white matters on ventral white matter (VWM) and lateral white matter (LWM). The percentage of spared white matters area decreased to the lowest 3 days after inju-ry, and reached a platform from 7 to 28 days after injury. The number of NF200-IR axons reduced significantly in white matter tracts after SCI. Conclusion Ventral spared white matters plays a key role in the recovery of motor function in rat with SCI, and the first 3 days was a time window to protect the white matters from injury.