南方水产科学
南方水產科學
남방수산과학
SOUTH CHINA FISHERIES SCIENCE
2015年
2期
50-56
,共7页
韩萍%杨丽诗%吴松%杨其彬%黄建华%周发林%江世贵
韓萍%楊麗詩%吳鬆%楊其彬%黃建華%週髮林%江世貴
한평%양려시%오송%양기빈%황건화%주발림%강세귀
促性腺激素释放激素%地欧酮%组织培养%卵巢发育%斑节对虾%卵巢自发荧光
促性腺激素釋放激素%地歐酮%組織培養%卵巢髮育%斑節對蝦%卵巢自髮熒光
촉성선격소석방격소%지구동%조직배양%란소발육%반절대하%란소자발형광
GnRH%domperidone%tissue culture%ovarian maturation%P. monodon%autofluorescence
研究了中华绒螯蟹（ Eriocheir sinensis）促性腺激素释放激素（ EsGnRH-I）、克氏原螯虾（ Procambarus clarkii）促性腺激素释放激素（PcGnRH）、多巴胺拮抗物地欧酮（DOM）及神经组织对斑节对虾（Penaeus monodon）卵巢发育的调控作用效果。试验前使用石蜡切片、HE染色及荧光观察确定斑节对虾卵巢发育分期及各期卵母细胞形态特征。结果显示，斑节对虾卵巢具有自发荧光现象，主要集中于滤泡细胞以及富含卵黄颗粒的次级卵母细胞、成熟卵母细胞的细胞质区域，荧光强度与卵巢发育阶段具有显著正相关性（ P﹤0.01）。随后采用离体卵巢组织培养的方法研究3种激素对卵巢发育的影响。结果表明，50 ng·mL-1 EsGnRH-I、500 ng·mL-1 PcGnRH和0.3μg· mL-1 DOM 对离体卵巢的发育有明显的刺激作用，显著增大卵母细胞直径（ P﹤0.01），与脑组织、胸神经处理组效果相似；同时组合使用 PcGnRH和 DOM 能极显著促进卵母细胞发育（ P ﹤0.01）。荧光定量 PCR 结果显示PcGnRH和DOM处理后能显著增加卵黄蛋白原的积累量。说明甲壳类动物GnRH和DOM均能促进斑节对虾卵母细胞成熟，并具有协同作用。
研究瞭中華絨螯蟹（ Eriocheir sinensis）促性腺激素釋放激素（ EsGnRH-I）、剋氏原螯蝦（ Procambarus clarkii）促性腺激素釋放激素（PcGnRH）、多巴胺拮抗物地歐酮（DOM）及神經組織對斑節對蝦（Penaeus monodon）卵巢髮育的調控作用效果。試驗前使用石蠟切片、HE染色及熒光觀察確定斑節對蝦卵巢髮育分期及各期卵母細胞形態特徵。結果顯示，斑節對蝦卵巢具有自髮熒光現象，主要集中于濾泡細胞以及富含卵黃顆粒的次級卵母細胞、成熟卵母細胞的細胞質區域，熒光彊度與卵巢髮育階段具有顯著正相關性（ P﹤0.01）。隨後採用離體卵巢組織培養的方法研究3種激素對卵巢髮育的影響。結果錶明，50 ng·mL-1 EsGnRH-I、500 ng·mL-1 PcGnRH和0.3μg· mL-1 DOM 對離體卵巢的髮育有明顯的刺激作用，顯著增大卵母細胞直徑（ P﹤0.01），與腦組織、胸神經處理組效果相似；同時組閤使用 PcGnRH和 DOM 能極顯著促進卵母細胞髮育（ P ﹤0.01）。熒光定量 PCR 結果顯示PcGnRH和DOM處理後能顯著增加卵黃蛋白原的積纍量。說明甲殼類動物GnRH和DOM均能促進斑節對蝦卵母細胞成熟，併具有協同作用。
연구료중화융오해（ Eriocheir sinensis）촉성선격소석방격소（ EsGnRH-I）、극씨원오하（ Procambarus clarkii）촉성선격소석방격소（PcGnRH）、다파알길항물지구동（DOM）급신경조직대반절대하（Penaeus monodon）란소발육적조공작용효과。시험전사용석사절편、HE염색급형광관찰학정반절대하란소발육분기급각기란모세포형태특정。결과현시，반절대하란소구유자발형광현상，주요집중우려포세포이급부함란황과립적차급란모세포、성숙란모세포적세포질구역，형광강도여란소발육계단구유현저정상관성（ P﹤0.01）。수후채용리체란소조직배양적방법연구3충격소대란소발육적영향。결과표명，50 ng·mL-1 EsGnRH-I、500 ng·mL-1 PcGnRH화0.3μg· mL-1 DOM 대리체란소적발육유명현적자격작용，현저증대란모세포직경（ P﹤0.01），여뇌조직、흉신경처리조효과상사；동시조합사용 PcGnRH화 DOM 능겁현저촉진란모세포발육（ P ﹤0.01）。형광정량 PCR 결과현시PcGnRH화DOM처리후능현저증가란황단백원적적루량。설명갑각류동물GnRH화DOM균능촉진반절대하란모세포성숙，병구유협동작용。
This study investigated the effects of Eriocheir sinensis GnRH( EsGnRH-I ), Procambarus clarkii GnRH( PcGnRH ), domperidone( DOM)and nerve tissues on ovarian development in Penaeus monodon. The stage of ovarian and the oocyte morphology in each period were determined by paraffin sections,HE staining and fluorescence observation. The results show that P. monodon ovaries had natural autofluorescence. The fluorescence focused on the region including follicular cells,cytoplasm of developing oocyte and ma-ture oocyte which contained amount of yolk globules. The fluorescence intensity was positively correlated to ovarian developmental sta-ges(P﹤0. 01). Then we analyzed the effects of three types of hormones on the ovarian development by ovarian tissues cultured in vitro. The results showed that 50 ng·mL-1 EsGnRH-I,500 ng·mL-1 PcGnRH and 0. 3 μg·mL-1 DOM significantly increased the di-ameter of the oocytes(P ﹤0. 01),similar to the treatments of brain and thoracic nerve. Combined use of PcGnRH and DOM signifi-cantly increased the diameter of the oocytes(P ﹤0. 01). Real time quantitative PCR show that treatments of GnRH and DOM obvious-ly induced the accumulation of vitellogenin. In conclusion, crustaceans GnRH and DOM could promote the oocyte maturation of P. monodon,and combined use of them had asynergistic effect.
