CAJ | 학술논문

目的：评价BacT／ALERT 3D系统在诊断结核性脑膜炎中的应用价值。方法：收集2009年1月～2014年6月间200例结核性脑膜炎患者早期脑脊液标本480份，每份标本分别用离心涂片法、罗氏培养法和BacT／ALERT 3D系统培养法检测，对其结果进行分析，总结3种方法对确诊结核性脑膜炎之间的差异。结果：480份脑脊液标本中，离心涂片法、罗氏培养法、BacT／ALERT 3D系统培养法的结核杆菌阳性检出率分别为3．13％、5．42％、10．63％，BacT／ALERT 3D系统培养法结核杆菌初代分离时间短于罗氏培养法。结论：BacT／ALERT 3D系统有较高的检出率、较快的检出时间，有助于提高结核性脑膜炎的发现率，值得临床推广应用。
목적：평개BacT／ALERT 3D계통재진단결핵성뇌막염중적응용개치。방법：수집2009년1월～2014년6월간200례결핵성뇌막염환자조기뇌척액표본480빈，매빈표본분별용리심도편법、라씨배양법화BacT／ALERT 3D계통배양법검측，대기결과진행분석，총결3충방법대학진결핵성뇌막염지간적차이。결과：480빈뇌척액표본중，리심도편법、라씨배양법、BacT／ALERT 3D계통배양법적결핵간균양성검출솔분별위3．13％、5．42％、10．63％，BacT／ALERT 3D계통배양법결핵간균초대분리시간단우라씨배양법。결론：BacT／ALERT 3D계통유교고적검출솔、교쾌적검출시간，유조우제고결핵성뇌막염적발현솔，치득림상추엄응용。
Objective:To evaluate the application value of BacT/ALERT 3D microbial detection system in rapid diagnosis of tuberculosis meningitis . Methods:480 specimens in cerebrospinsl fluid ( CSF) of 200 patients diagnosed with tuberculosis meningitis were collected from January 2009 to June 2014.These specimens were processed for detection of the mycobacterium tuberculosis by centrifugal smear acid-fast stain ( Ziehl-Neelsen staining) and Lowenstein-Jensen cultivation,as well as the BacT/ALERT 3D system.The above results were analyzed and compared regarding the positive rates in order to evaluate the value in diagnosing tuberculosis meningitis .Results:The positive rates of the centrifugal smear acid-fast smear,Lowenstein-Jensen cultiva-tion and BacT/ALERT 3D system cultivation were 3.13%,5.42% and 10.63% respectively.Conclusion: BacT/Alert 3D microbial detection system may lead to higher sensitivity and shortened detection time ,which is worthy of wider clinical recommendation .