皖南医学院学报
皖南醫學院學報
환남의학원학보
ACTA ACADEMIAE MEDICINAE WANNAN
2015年
2期
140-143
,共4页
齐晶%徐又海%汪兴洪
齊晶%徐又海%汪興洪
제정%서우해%왕흥홍
Pim-1%C-myc%原癌基因%多发性骨髓瘤%RT-PCR
Pim-1%C-myc%原癌基因%多髮性骨髓瘤%RT-PCR
Pim-1%C-myc%원암기인%다발성골수류%RT-PCR
Pim-1%c-myc%proto-oncogene%multiple myeloma%RT-PCR
目的：探讨莫罗尼鼠白血病病毒前病毒整合基因-1（ proviral integration of moloney murine leukemia virus ，Pim-1）及C-myc在初诊多发性骨髓瘤（ multiple myeloma ，MM）发病机制中的可能作用。方法：采用RT-PCR检测22例MM患者骨髓及10例非恶性血液病患者骨髓中Pim-1 mRNA及C-myc mRNA的表达水平。结果：在初诊的多发性骨髓瘤患者中Pim-1和C-myc基因的表达显著高于对照组，二者比较有差异性（ P＜0.05）；Pim-1和 C-myc 基因的表达成正相关（ r＝0.577， P＜0.01）。结论：Pim-1及C-myc基因的过度表达，在多发性髓瘤的发生发展中可能具有协同作用。
目的：探討莫囉尼鼠白血病病毒前病毒整閤基因-1（ proviral integration of moloney murine leukemia virus ，Pim-1）及C-myc在初診多髮性骨髓瘤（ multiple myeloma ，MM）髮病機製中的可能作用。方法：採用RT-PCR檢測22例MM患者骨髓及10例非噁性血液病患者骨髓中Pim-1 mRNA及C-myc mRNA的錶達水平。結果：在初診的多髮性骨髓瘤患者中Pim-1和C-myc基因的錶達顯著高于對照組，二者比較有差異性（ P＜0.05）；Pim-1和 C-myc 基因的錶達成正相關（ r＝0.577， P＜0.01）。結論：Pim-1及C-myc基因的過度錶達，在多髮性髓瘤的髮生髮展中可能具有協同作用。
목적：탐토막라니서백혈병병독전병독정합기인-1（ proviral integration of moloney murine leukemia virus ，Pim-1）급C-myc재초진다발성골수류（ multiple myeloma ，MM）발병궤제중적가능작용。방법：채용RT-PCR검측22례MM환자골수급10례비악성혈액병환자골수중Pim-1 mRNA급C-myc mRNA적표체수평。결과：재초진적다발성골수류환자중Pim-1화C-myc기인적표체현저고우대조조，이자비교유차이성（ P＜0.05）；Pim-1화 C-myc 기인적표체성정상관（ r＝0.577， P＜0.01）。결론：Pim-1급C-myc기인적과도표체，재다발성수류적발생발전중가능구유협동작용。
Objective:To explore the mechanisms of proviral integration site for moloney murine leukemia virus 1(Pim-1) and c-myc in the pathogenesis of multiple myeloma(MM).Methods:Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the Pim-1 mRNA and c-myc mRNA level in the bone marrow specimens from 22 newly diagnosed MM cases and 10 cases of non-hematological malignancies.Results:The level of Pim-1 mRNA and c-myc mRNA in newly diagnosed MM cases was significantly higher than that of control group(P<0.05),and the Pim-1 mRNA expression was posi-tively correlated with c-myc mRNA(r=0.577,P<0.01).Conclusion:Over-expression of Pim-1 and c-myc in MM indicates that the two proto-oncogenes may be synergetic effect in the pathogenesis and progression of this entity .
