CAJ | 학술논문

目的：通过对胃癌组织及癌旁组织中miRNA-449b表达的比较，分析miRNA-449b与胃癌发生及淋巴结转移的关系，为进一步探讨其在胃癌中的作用机制提供依据。方法：收集30例胃癌及癌旁组织标本，提取组织总RNA；茎环法反转录成cDNA；用实时荧光定量PCR方法检测miRNA-449b在组织中的表达水平。结果：相比于癌旁组织，miRNA-449b在胃癌组织中有23例（76．67％）表达下调，7例（23．33％）表达上调，其平均表达水平低于癌旁组织，差异有统计学意义（ t＝2．395，P＝0．023，P＜0．05）。 miRNA-449b在17例有淋巴结转移的样本中16例表达下调（94．12％），13例无淋巴结转移的样本只有7例表达下调（53．85％），淋巴结转移组与无淋巴结转移组miRNA-449b的下调比例，经卡方检验，差异有统计学意义（P＝0．025，P＜0．05）。结论：miRNA-449b与胃癌的发生及淋巴结转移相关。
목적：통과대위암조직급암방조직중miRNA-449b표체적비교，분석miRNA-449b여위암발생급림파결전이적관계，위진일보탐토기재위암중적작용궤제제공의거。방법：수집30례위암급암방조직표본，제취조직총RNA；경배법반전록성cDNA；용실시형광정량PCR방법검측miRNA-449b재조직중적표체수평。결과：상비우암방조직，miRNA-449b재위암조직중유23례（76．67％）표체하조，7례（23．33％）표체상조，기평균표체수평저우암방조직，차이유통계학의의（ t＝2．395，P＝0．023，P＜0．05）。 miRNA-449b재17례유림파결전이적양본중16례표체하조（94．12％），13례무림파결전이적양본지유7례표체하조（53．85％），림파결전이조여무림파결전이조miRNA-449b적하조비례，경잡방검험，차이유통계학의의（P＝0．025，P＜0．05）。결론：miRNA-449b여위암적발생급림파결전이상관。
Objective:To investigate the expression of miRNA-449b in gastric cancer and its adjacent tissue and incidence of lymph node metastasis for elucidating the mechanisms of this gene in development of gastric cancer .Methods: The specimen together with adjacent tissues was obtained in 30 pa-tients of gastric carcinoma.Total RNA was extracted from these tissues,and reversely transcribed into cDNA by stem-loop technique.Real-time fluorescence quota PCR was used to determine miRNA-449b expression in the tissues.Results:Expression of miRNA-449b was down-regulated in 23 cases (76.67%) and up-regulated in 7 (23.33%).The expression level was generally lower than that of the adjacent tissues(t=2.395,P=0.023,P<0.05).The miR-NA-449b expression was down-regulated in 16 of 17 cases with lymph node metastasis (94.12%),and in 7 of 13 without metastasis(53.85%),which suggested significant difference by Chi-square test (P=0.025,P<0.05).Conclusion: miR-449b is associated with the development of gastric cancer and presence of lymph node metastasis.