亚热带植物科学
亞熱帶植物科學
아열대식물과학
SUBTROPICAL PLANT SCIENCE
2015年
1期
1-7
,共7页
杨凤娇%李红英%卢存福%陈玉珍
楊鳳嬌%李紅英%盧存福%陳玉珍
양봉교%리홍영%로존복%진옥진
玉米%自交系%化学诱变%SSR标记%遗传变异
玉米%自交繫%化學誘變%SSR標記%遺傳變異
옥미%자교계%화학유변%SSR표기%유전변이
maize%inbred lines%chemical mutagenesis%SSR markers%genetic variation
以玉米基础自交系(AS-9)及其化学诱变自交系M4为实验材料进行耐盐性鉴定，同时利用44对可能与根系性状有关联的SSR引物进行遗传变异分析。结果表明，诱变M4幼苗经200和250 mmol·L-1 NaCl盐胁迫处理7 d后，IV400、IV1600根数比AS-9对照分别增加23.1%、19.1%，诱变IV1600初生根1、2、3根长分别为对照的1.33、1.70、2.15倍；同时在M4玉米试材中扩增出多态性条带的引物25对，占引物总数的56.82%；诱变自交系M4与AS-9的遗传相似系数平均值为0.6563，遗传相似系数中心化后，数据在-0.04处可把材料分为三个类群，基础材料AS-9为Ⅰ类，M4代IV1400、IV1600、IV2000聚为Ⅲ类，其余诱变系为Ⅱ类。此外，6对引物的SSR-PCR产物进行序列差异检测，结果产生新的基元和基元重复次数变化，说明系列化学诱变已使玉米自交系AS-9产生了显著的遗传变异。
以玉米基礎自交繫(AS-9)及其化學誘變自交繫M4為實驗材料進行耐鹽性鑒定，同時利用44對可能與根繫性狀有關聯的SSR引物進行遺傳變異分析。結果錶明，誘變M4幼苗經200和250 mmol·L-1 NaCl鹽脅迫處理7 d後，IV400、IV1600根數比AS-9對照分彆增加23.1%、19.1%，誘變IV1600初生根1、2、3根長分彆為對照的1.33、1.70、2.15倍；同時在M4玉米試材中擴增齣多態性條帶的引物25對，佔引物總數的56.82%；誘變自交繫M4與AS-9的遺傳相似繫數平均值為0.6563，遺傳相似繫數中心化後，數據在-0.04處可把材料分為三箇類群，基礎材料AS-9為Ⅰ類，M4代IV1400、IV1600、IV2000聚為Ⅲ類，其餘誘變繫為Ⅱ類。此外，6對引物的SSR-PCR產物進行序列差異檢測，結果產生新的基元和基元重複次數變化，說明繫列化學誘變已使玉米自交繫AS-9產生瞭顯著的遺傳變異。
이옥미기출자교계(AS-9)급기화학유변자교계M4위실험재료진행내염성감정，동시이용44대가능여근계성상유관련적SSR인물진행유전변이분석。결과표명，유변M4유묘경200화250 mmol·L-1 NaCl염협박처리7 d후，IV400、IV1600근수비AS-9대조분별증가23.1%、19.1%，유변IV1600초생근1、2、3근장분별위대조적1.33、1.70、2.15배；동시재M4옥미시재중확증출다태성조대적인물25대，점인물총수적56.82%；유변자교계M4여AS-9적유전상사계수평균치위0.6563，유전상사계수중심화후，수거재-0.04처가파재료분위삼개류군，기출재료AS-9위Ⅰ류，M4대IV1400、IV1600、IV2000취위Ⅲ류，기여유변계위Ⅱ류。차외，6대인물적SSR-PCR산물진행서렬차이검측，결과산생신적기원화기원중복차수변화，설명계렬화학유변이사옥미자교계AS-9산생료현저적유전변이。
Chemical mutation was an effective way of creating new germplasm resources. To analyze the impact of chemical mutagenesis on AS-9 maize inbred offspring, the salt tolerance and genetic variation between mutant M4 and the corresponding original material (AS-9) were evaluated by using 44 pairs of SSR markers that may be associated with root traits. Seed germination, bud length and root number of M4 were higher than that of AS-9. After treatment with 200 and 250 mmol·L-1 NaCl for 7 days, root numbers of IV400 and IV1600 seedlings were higher than that of AS-9, with increases of 23.1% and 19.1%, respectively. IV1600 primary root lengths of 1, 2, 3 were 1.33, 1.70, and 2.15 times longer than that of AS-9, respectively. Twenty five pairs of SSR primers gave profiles amplified in sample of M4mutants, accounting for 56.82% of the total pairs of primers. The average value of genetic similarity coefficient between IVCK and M4 was 0.6563. With the centered genetic similarity coefficient of-0.04 by UPGMA, the maize inbred line materials were divided into three groups: the original material (IVCK), the M4 mutants including IV1400, IV1600, IV2000, and other M4 mutants (IV200, IV400, IV800, IV1200). Principal component analysis and clustering results were similar. In addition, SSR-PCR products amplified by six pairs of primers were sequenced, the results showed that there was distinct variation between AS-9 and the induced M4, indicating chemical mutagenesis made maize inbred AS-9 produce a wide range of genetic variation.
