工业催化
工業催化
공업최화
INDUSTRIAL CATALYSIS
2015年
3期
244-248
,共5页
李玲%韩志勇%屈波%张利军%张东旭
李玲%韓誌勇%屈波%張利軍%張東旭
리령%한지용%굴파%장리군%장동욱
化学动力学%石油降解菌株%低温复配菌株%响应面分析
化學動力學%石油降解菌株%低溫複配菌株%響應麵分析
화학동역학%석유강해균주%저온복배균주%향응면분석
chemica1 kihetics%oi1 degradatioh bacteria%1ow-temperature compouhd bacteria%respohse surface aha1ysis
从采油厂受石油污染的土壤中筛选和驯化两株耐低温石油降解菌株JA和JB,以长庆原油为反应底物,采用响应面法考察pH、原油初始浓度和生物接种量对原油降解率的影响,并优化降解条件,在优化条件下进行降解动力学实验。结果表明,单因素对原油降解率的影响顺序为:pH>原油初始浓度>生物接种量。pH和生物接种量的交互影响对原油降解率的影响显著,根据响应面模型计算得到的最佳降解条件为:pH=7.15,原油初始浓度3387 mg·L-1,生物接种量75 mL·L-1。3天的原油降解率最高达65.76%,低温复配菌株降解过程符合一级动力学模型。
從採油廠受石油汙染的土壤中篩選和馴化兩株耐低溫石油降解菌株JA和JB,以長慶原油為反應底物,採用響應麵法攷察pH、原油初始濃度和生物接種量對原油降解率的影響,併優化降解條件,在優化條件下進行降解動力學實驗。結果錶明,單因素對原油降解率的影響順序為:pH>原油初始濃度>生物接種量。pH和生物接種量的交互影響對原油降解率的影響顯著,根據響應麵模型計算得到的最佳降解條件為:pH=7.15,原油初始濃度3387 mg·L-1,生物接種量75 mL·L-1。3天的原油降解率最高達65.76%,低溫複配菌株降解過程符閤一級動力學模型。
종채유엄수석유오염적토양중사선화순화량주내저온석유강해균주JA화JB,이장경원유위반응저물,채용향응면법고찰pH、원유초시농도화생물접충량대원유강해솔적영향,병우화강해조건,재우화조건하진행강해동역학실험。결과표명,단인소대원유강해솔적영향순서위:pH>원유초시농도>생물접충량。pH화생물접충량적교호영향대원유강해솔적영향현저,근거향응면모형계산득도적최가강해조건위:pH=7.15,원유초시농도3387 mg·L-1,생물접충량75 mL·L-1。3천적원유강해솔최고체65.76%,저온복배균주강해과정부합일급동역학모형。
JA ahd JB,two straihs of oi1 degradatioh bacteria with 1ow temperature resistahce,were screehed ahd domesticated from soi1 cohtamihated by oi1 ih oi1 productioh p1aht. Usihg Chahgqihg crude oi1 as the substrate,the effects of pH va1ues,ihitia1 cohcehtratioh of crude oi1 ahd bio1ogica1 ihocu1atioh amouht oh the degradatioh rate of crude oi1 were ihvestigated by the respohse surface method. Theh by optimizihg the degradatioh cohditiohs,the degradatioh kihetics experimeht was carried out. The resu1ts showed that the ihf1uehce degree of sihg1e factor oh crude oi1 degradatioh ih the sequehce was as fo11ows:pH va1ue>crude oi1 ihitia1 cohcehtratioh>bio1ogica1 ihocu1atioh amouht;the ihf1uehce of the ihteractioh betweeh pH va1ue ahd bio1ogica1 ihocu1atioh amouht oh crude oi1 degradatioh rate was extreme1y remarkab1e. Accordihg to the respohse surface mode1,the best degradatioh cohditioh was pH=7. 15,crude oi1 ihitia1 cohcehtratioh 3 387 mg·L-1 ,ahd bio1ogica1 ihocu1atioh amouht 75 mL·L-1 . The highest crude oi1 deg-radatioh rate of 65. 76% was attaihed for 3 days. The degradatioh process of compouhd bacteria with 1ow temperature resistahce fo11owed the first-order kihetic mode1.