CAJ | 학술논문

目的：探讨B细胞转录激活因子(BATF)、白细胞介素-17及维甲酸孤儿核受体γt (RORγt)对急性哮喘小鼠气道炎症的影响。方法(1)将24只健康雌性BALB/c小鼠随机分为生理盐水对照组(NS组)、哮喘模型组(AS组)、地塞米松治疗组(DEX组)，每组8只。第0、7、14天，AS组、DEX组小鼠用卵清蛋白(OVA)抗原液腹腔内注射致敏，NS组用等体积生理盐水腹腔内注射。第21天起，NS组超声雾化吸入生理盐水，AS组、DEX组超声雾化吸入2%的OVA液，均为每天1次，每次40 min，连续5 d。每次雾化前30 min，DEX组以地塞米松1.0 mg/kg腹腔注射， NS组、AS组则以等体积的生理盐水腹腔注射。末次雾化结束24 h后，各组小鼠留取肺组织标本进行检测。结果(1)肺组织切片显示：NS组肺组织无明显炎症反应，AS组及DEX组均存在炎症反应，且AS组炎症反应明显高于DEX组。(2)免疫组化结果显示：AS组肺组织BATF、IL-17、RORγt的表达明显高于NS组、DEX组(P<0.05)；DEX组肺组织IL-17、BATF、RORγt的表达较AS组明显降低(P<0.05)。(3)肺组织BATF的表达与BALF中白细胞(WBC)总数及中性粒细胞(NEU)计数、嗜酸性粒细胞(EOS)计数呈正相关(r=0.487、0.531、0.515，P<0.05)；肺组织BATF的表达与IL-17、RORγt的表达呈正相关(r=0.502、0.493，P<0.05)；肺组织IL-17的表达与RORγt的表达呈正相关(r=0.536，P<0.05)。结论(1)急性哮喘小鼠肺组织中BATF、IL-17、RORγt表达上调。(2)地塞米松可能是通过下调BATF、IL-17、RORγt的表达而减轻气道炎症性损害。
목적：탐토B세포전록격활인자(BATF)、백세포개소-17급유갑산고인핵수체γt (RORγt)대급성효천소서기도염증적영향。방법(1)장24지건강자성BALB/c소서수궤분위생리염수대조조(NS조)、효천모형조(AS조)、지새미송치료조(DEX조)，매조8지。제0、7、14천，AS조、DEX조소서용란청단백(OVA)항원액복강내주사치민，NS조용등체적생리염수복강내주사。제21천기，NS조초성무화흡입생리염수，AS조、DEX조초성무화흡입2%적OVA액，균위매천1차，매차40 min，련속5 d。매차무화전30 min，DEX조이지새미송1.0 mg/kg복강주사， NS조、AS조칙이등체적적생리염수복강주사。말차무화결속24 h후，각조소서류취폐조직표본진행검측。결과(1)폐조직절편현시：NS조폐조직무명현염증반응，AS조급DEX조균존재염증반응，차AS조염증반응명현고우DEX조。(2)면역조화결과현시：AS조폐조직BATF、IL-17、RORγt적표체명현고우NS조、DEX조(P<0.05)；DEX조폐조직IL-17、BATF、RORγt적표체교AS조명현강저(P<0.05)。(3)폐조직BATF적표체여BALF중백세포(WBC)총수급중성립세포(NEU)계수、기산성립세포(EOS)계수정정상관(r=0.487、0.531、0.515，P<0.05)；폐조직BATF적표체여IL-17、RORγt적표체정정상관(r=0.502、0.493，P<0.05)；폐조직IL-17적표체여RORγt적표체정정상관(r=0.536，P<0.05)。결론(1)급성효천소서폐조직중BATF、IL-17、RORγt표체상조。(2)지새미송가능시통과하조BATF、IL-17、RORγt적표체이감경기도염증성손해。
Objective To study the role of the B cell activating transcription factor (BATF), IL-17 and the retino-ic acid-related orphan nuclear receptor (RORγt) in acute airway inflammation of asthmatic mice. Methods (1) Twen-ty-four healthy female BALB/c mice were randomly divided into the normal saline group (NS group), the asthma group (AS group), and the dexamethasone group (DEX group), with 8 mice in each group. On days 0, 7 and 14, oval-bumin (OVA) fluid was injected into the abdominal cavity of the mice in AS group and DEX group, and normal saline was injected into the abdominal cavity of the mice in NS group. From the 21st day, the mice in NS group were inhaled normal saline with 40 minutes of ultrasonic atomization once a day for five consecutive days, while the mice in AS group and DEX group were inhaled 2%OVA solution in the same way. The mice in DEX group were given intraperito-neal injection of dexamethasone (1.0 mg/kg) at 30 minutes before the inhalation each time, and the mice in other groups were given the normal saline group in the same way. 24 hours after the final inhalation, mice were collected the lung tissue samples for testing. Results (1) According to lung tissue sections, lung tissue in NS group had no signifi-cant inflammation, while the lung tissue in AS and DEX groups showed inflammation response. AS group's inflamma-tory response was higher than DEX group's. (2) Immunohistochemistry results showed the expression of BATF, IL-17 and RORγt in AS group were higher than that in NS group and DEX group (P<0.05), and the expression in DEX group was significantly lower than the AS group (P<0.05). (3) The expression of BATF in lung tissue was positively correlated with the count of WBC, NEU and EOS in the BALF differently (r=0.487, 0.531, 0.515, P<0.05). The ex-pression of BATF in lung tissue was positively correlated with the one of RORγt and IL-17 differently (r=0.502, 0.493, P<0.05). The expression of IL-17 in lung tissue was positively correlated with the one of RORγt (r=0.536, P<0.05). Conclusion (1) The expression of BATF, IL-17 and RORγt in AS group's lung tissue was increased signifi-cantly. (2) Dexamethasone could down-regulate the expression of BATF, IL-17 and RORγt in asthmatic mice, which would improve airway inflammatory.