海南医学
海南醫學
해남의학
HAINAN MEDICAL JOURNAL
2015年
7期
1001-1004
,共4页
高慧%何金花%崔美玲%陈伟明%潘莲娣
高慧%何金花%崔美玲%陳偉明%潘蓮娣
고혜%하금화%최미령%진위명%반련제
肝炎病毒%乙型%测序%X基因%基因型%变异%肝硬化%肝癌
肝炎病毒%乙型%測序%X基因%基因型%變異%肝硬化%肝癌
간염병독%을형%측서%X기인%기인형%변이%간경화%간암
Hepatitis virus%Hepatitis B%Sequencing%X gene%Genotype%Variation%Liver cirrhosis%Hepato-carcinoma
目的:对乙型肝炎病毒(HBV)感染者血清标本的HBV X基因进行测序,以了解其突变情况。方法从广州市番禺区133例HBV感染者的血清标本中提取HBV DNA,以PCR方法扩增HBV X基因,再进行测序,根据患者不同病程将其分为乙肝携带组(ASC组)36例、慢性肝炎组(CHB组)40例、肝硬化组(LC组)34例和肝癌组(HCC组)23例,并对结果进行综合比较分析。结果人均碱基突变数ASC组为(5.17±0.86)、CHB组为(5.05±0.80)、LC组为(3.43±0.59),HCC组为(2.05±0.44),ASC组、CHB组和LC组比较差异均无统计学意义(P>0.05),而HCC组均低于ASC组、CHB组、LC组,差异均有统计学意义(P<0.05);HBV X基因突变检出率排前4位的位点是:A1762T联合G1764A双变异为85例(63.91%),T1719G联合G1721A同时变异为77例(57.89%),T1544A为62例(46.62%),T1753C为45例(33.83%)。前4位突变检出率均在LC组中最高(P<0.05)。结论对HBV X基因的高突变位点进行检测为肝硬化及肝癌的发病机制提供线索,对尽早预防和预测肝硬化及肝癌具有重要意义。
目的:對乙型肝炎病毒(HBV)感染者血清標本的HBV X基因進行測序,以瞭解其突變情況。方法從廣州市番禺區133例HBV感染者的血清標本中提取HBV DNA,以PCR方法擴增HBV X基因,再進行測序,根據患者不同病程將其分為乙肝攜帶組(ASC組)36例、慢性肝炎組(CHB組)40例、肝硬化組(LC組)34例和肝癌組(HCC組)23例,併對結果進行綜閤比較分析。結果人均堿基突變數ASC組為(5.17±0.86)、CHB組為(5.05±0.80)、LC組為(3.43±0.59),HCC組為(2.05±0.44),ASC組、CHB組和LC組比較差異均無統計學意義(P>0.05),而HCC組均低于ASC組、CHB組、LC組,差異均有統計學意義(P<0.05);HBV X基因突變檢齣率排前4位的位點是:A1762T聯閤G1764A雙變異為85例(63.91%),T1719G聯閤G1721A同時變異為77例(57.89%),T1544A為62例(46.62%),T1753C為45例(33.83%)。前4位突變檢齣率均在LC組中最高(P<0.05)。結論對HBV X基因的高突變位點進行檢測為肝硬化及肝癌的髮病機製提供線索,對儘早預防和預測肝硬化及肝癌具有重要意義。
목적:대을형간염병독(HBV)감염자혈청표본적HBV X기인진행측서,이료해기돌변정황。방법종엄주시번우구133례HBV감염자적혈청표본중제취HBV DNA,이PCR방법확증HBV X기인,재진행측서,근거환자불동병정장기분위을간휴대조(ASC조)36례、만성간염조(CHB조)40례、간경화조(LC조)34례화간암조(HCC조)23례,병대결과진행종합비교분석。결과인균감기돌변수ASC조위(5.17±0.86)、CHB조위(5.05±0.80)、LC조위(3.43±0.59),HCC조위(2.05±0.44),ASC조、CHB조화LC조비교차이균무통계학의의(P>0.05),이HCC조균저우ASC조、CHB조、LC조,차이균유통계학의의(P<0.05);HBV X기인돌변검출솔배전4위적위점시:A1762T연합G1764A쌍변이위85례(63.91%),T1719G연합G1721A동시변이위77례(57.89%),T1544A위62례(46.62%),T1753C위45례(33.83%)。전4위돌변검출솔균재LC조중최고(P<0.05)。결론대HBV X기인적고돌변위점진행검측위간경화급간암적발병궤제제공선색,대진조예방화예측간경화급간암구유중요의의。
Objective To understand the mutation of hepatitis B virus X gene by gene sequencing from HBV infected serum. Methods HBV DNAs were extracted from the serum of 133 infectors from Panyu area, and HBV X genes were amplified by PCR and then sequenced. According to the different courses of disease, patients were divided into four groups:36 cases of hepatitis B virus carrier (ASC), 40 cases of chronic hepatitis (CHB), 34 cases of liv-er cirrhosis (LC) and 23 cases of hepatocellular carcinoma (HCC). And the results were comprehensively and compara-tively analyzed. Results The mutation numbers per capita of four groups were as follows:ASC group (5.17 ± 0.86), CHB group (5.05±0.80), LC group (3.43±0.86) and HCC group (2.05±0.44). And there were no statistically significant difference between the ASC group, CHB group and LC group (P>0.05), while the number in HCC group was lower than that in the other three groups (P<0.05). The top 4 sites of HBV X gene mutation detection rates were:85 cases of A1762T/G1764A double mutation (63.91%), 77 cases of T1719G/G1721A simultaneous mutation (57.89%), 62 cases of T1544A (46.62%) and 45 cases of T1753C (33.83%). The top 4 sites of mutation detection rates were all highest in LC group (P<0.05). Conclusion Detection of the high mutation site of HBV X gene could help provide clues for study-ing the mechanism of liver cirrhosis and liver cancer, which also presents significant reference for clinical practice.