天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2015年
4期
344-347,450
,共5页
甄珍%蒋少云%陶玉飞%严志敏%邓嘉胤
甄珍%蔣少雲%陶玉飛%嚴誌敏%鄧嘉胤
견진%장소운%도옥비%엄지민%산가윤
成纤维细胞生长因子2%成纤维细胞%牙龈%骨生成%细胞分化%细胞增殖
成纖維細胞生長因子2%成纖維細胞%牙齦%骨生成%細胞分化%細胞增殖
성섬유세포생장인자2%성섬유세포%아간%골생성%세포분화%세포증식
fibroblast growth factor 2%fibroblasts%gingiva%osteogenesis%cell differentiation%cell proliferation
目的：观察碱性成纤维细胞生长因子（bFGF）对体外培养的人牙龈成纤维细胞（HGFs）成骨分化能力与细胞增殖的影响，探索bFGF在HGFs体外诱导成骨分化过程中的作用。方法采用组织块贴壁法体外培养HGFs，取第3代细胞进行如下分组培养。1组为普通培养基组，2组为普通培养基+10μg/L bFGF组，3组为成骨诱导组，4组为成骨诱导+10μg/L bFGF组。应用四甲基偶氮唑蓝比色法检测HGFs增殖状况；用碱性磷酸酶染色法及茜素红染色法检测HGFs的成骨分化能力。结果在普通培养基和成骨诱导培养基中，10μg/L的bFGF均能促进HGFs的增殖（P＜0.01）；在成骨诱导培养基中HGFs具有骨向分化能力，形成钙结节；而10μg/L的bFGF对HGFs的碱性磷酸酶活性与矿化结节形成能力均无明显影响。结论10μg/L的bFGF能促进HGFs的增殖能力，而对其骨向分化无明显影响。
目的：觀察堿性成纖維細胞生長因子（bFGF）對體外培養的人牙齦成纖維細胞（HGFs）成骨分化能力與細胞增殖的影響，探索bFGF在HGFs體外誘導成骨分化過程中的作用。方法採用組織塊貼壁法體外培養HGFs，取第3代細胞進行如下分組培養。1組為普通培養基組，2組為普通培養基+10μg/L bFGF組，3組為成骨誘導組，4組為成骨誘導+10μg/L bFGF組。應用四甲基偶氮唑藍比色法檢測HGFs增殖狀況；用堿性燐痠酶染色法及茜素紅染色法檢測HGFs的成骨分化能力。結果在普通培養基和成骨誘導培養基中，10μg/L的bFGF均能促進HGFs的增殖（P＜0.01）；在成骨誘導培養基中HGFs具有骨嚮分化能力，形成鈣結節；而10μg/L的bFGF對HGFs的堿性燐痠酶活性與礦化結節形成能力均無明顯影響。結論10μg/L的bFGF能促進HGFs的增殖能力，而對其骨嚮分化無明顯影響。
목적：관찰감성성섬유세포생장인자（bFGF）대체외배양적인아간성섬유세포（HGFs）성골분화능력여세포증식적영향，탐색bFGF재HGFs체외유도성골분화과정중적작용。방법채용조직괴첩벽법체외배양HGFs，취제3대세포진행여하분조배양。1조위보통배양기조，2조위보통배양기+10μg/L bFGF조，3조위성골유도조，4조위성골유도+10μg/L bFGF조。응용사갑기우담서람비색법검측HGFs증식상황；용감성린산매염색법급천소홍염색법검측HGFs적성골분화능력。결과재보통배양기화성골유도배양기중，10μg/L적bFGF균능촉진HGFs적증식（P＜0.01）；재성골유도배양기중HGFs구유골향분화능력，형성개결절；이10μg/L적bFGF대HGFs적감성린산매활성여광화결절형성능력균무명현영향。결론10μg/L적bFGF능촉진HGFs적증식능력，이대기골향분화무명현영향。
Objective To observe the effects of basic fibroblast growth factor (bFGF) on osteogenic differentiation abili?ty and cell proliferation of human gingival fibroblasts (HGFs), and to explore the role of bFGF on the process of osteogenic differencitiaion in vitro. Methods HGFs were cultured in vitro until the 3rd passage when they were divided into four groups:normal medium as group 1, normal medium with 10μg/L bFGF as group 2, osteogenic medium as group 3 and osteo?genic medium with 10μg/L bFGF as group 4. MTT assay was used to evaluate the proliferation of HGFs. Alkaline phospha?tase (ALP) staining and Alizarin red staining were applied to investigate osteogenic potential of HGFs under different culture conditions. Results bFGF at concentration of 10 μg/L could increase HGFs proliferation in both normal and osteogenic medium (P<0.01). HGFs could be induced towards osteogenic differentiation and form mineralized nodule in osteogenic me?dium. However, 10μg/L bFGF had no effects on ALP activity and mineralized nodule formation of HGFs during osteogenic differentiation. Conclusion bFGF could promote the proliferation of HGFs but show no effects on osteogenic differentiation of HGFs at concentration of 10μg/L.
