山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
16期
8-11
,共4页
姜黄素%甘草次酸-PEI-PLGA纳米粒%薄膜水化法%制备工艺
薑黃素%甘草次痠-PEI-PLGA納米粒%薄膜水化法%製備工藝
강황소%감초차산-PEI-PLGA납미립%박막수화법%제비공예
curcumin%glycyrrhetinic acid-PEI-PLGA nanoparticles%thin film hydration method%preparation process
目的：制备姜黄素（Cur）-甘草次酸（GA）-PEI-PLGA纳米粒，并优化其制备工艺。方法采用薄膜水化法制备Cur-GA-PEI-PLGA纳米粒，建立分析方法后，采用单因素和正交设计试验考察水化温度、水化时间、投药量对纳米粒包封率和载药量的影响，确定最佳制备条件。在最佳条件下制备Cur-GA-PEI-PLGA纳米粒，检测其包封率和载药量，采用透射电镜观察纳米粒的形态，动态光散射粒度分析仪测定纳米粒的粒径和zeta电位。结果 Cur-GA-PEI-PLGA纳米粒的最佳制备条件为水化温度60℃、水化时间3 h、投药量7 mg。在此条件下制备的纳米粒包封率为58．1％±1．2％，载药量为14．5％±1．4％；透射电镜下可见纳米粒米粒呈球形，大小均一；其平均粒径为330．3 nm，分布均匀（多分散系数为0．311）；zeta电位为39．2 mV。结论薄膜水化法适合用于Cur-GA-PEI-PLGA纳米粒的制备，最佳制备工艺为水化温度60℃、水化时间3 h、投药量7 mg。
目的：製備薑黃素（Cur）-甘草次痠（GA）-PEI-PLGA納米粒，併優化其製備工藝。方法採用薄膜水化法製備Cur-GA-PEI-PLGA納米粒，建立分析方法後，採用單因素和正交設計試驗攷察水化溫度、水化時間、投藥量對納米粒包封率和載藥量的影響，確定最佳製備條件。在最佳條件下製備Cur-GA-PEI-PLGA納米粒，檢測其包封率和載藥量，採用透射電鏡觀察納米粒的形態，動態光散射粒度分析儀測定納米粒的粒徑和zeta電位。結果 Cur-GA-PEI-PLGA納米粒的最佳製備條件為水化溫度60℃、水化時間3 h、投藥量7 mg。在此條件下製備的納米粒包封率為58．1％±1．2％，載藥量為14．5％±1．4％；透射電鏡下可見納米粒米粒呈毬形，大小均一；其平均粒徑為330．3 nm，分佈均勻（多分散繫數為0．311）；zeta電位為39．2 mV。結論薄膜水化法適閤用于Cur-GA-PEI-PLGA納米粒的製備，最佳製備工藝為水化溫度60℃、水化時間3 h、投藥量7 mg。
목적：제비강황소（Cur）-감초차산（GA）-PEI-PLGA납미립，병우화기제비공예。방법채용박막수화법제비Cur-GA-PEI-PLGA납미립，건립분석방법후，채용단인소화정교설계시험고찰수화온도、수화시간、투약량대납미립포봉솔화재약량적영향，학정최가제비조건。재최가조건하제비Cur-GA-PEI-PLGA납미립，검측기포봉솔화재약량，채용투사전경관찰납미립적형태，동태광산사립도분석의측정납미립적립경화zeta전위。결과 Cur-GA-PEI-PLGA납미립적최가제비조건위수화온도60℃、수화시간3 h、투약량7 mg。재차조건하제비적납미립포봉솔위58．1％±1．2％，재약량위14．5％±1．4％；투사전경하가견납미립미립정구형，대소균일；기평균립경위330．3 nm，분포균균（다분산계수위0．311）；zeta전위위39．2 mV。결론박막수화법괄합용우Cur-GA-PEI-PLGA납미립적제비，최가제비공예위수화온도60℃、수화시간3 h、투약량7 mg。
Objective To prepare curcumin (Cur)-glycyrrhetinic acid (GA)-PEI-PLGA nanoparticles and optimize the preparation process .Methods Cur-GA-PEI-PLGA nanoparticles were prepared by thin film hydration method .After establishing the analysis method , single factor experiment and orthogonal design experiment were used to evaluate the effects of hydration temperature , hydration time and drug addition on the entrapment efficiency and drug loading , and to determine the optimum preparation conditions .Cur-GA-PEI-PLGA nanoparticles were prepared under the optimum preparation condi-tions , and we determined the entrapment efficiency and drug loading .The morphology of the nanoparticles was observed by transmission electron microscope (TEM).The diameter and zeta potential of the nanoparticles were determined by dynamic light scattering particle size analyzer ( DLS) .Results The optimum preparation conditions of Cur-GA-PEI-PLGA nanopar-ticles were as follows:the hydration temperature was 60 ℃, the hydration time was 3 h, and the drug addition was 7 mg. The entrapment efficiency and drug loading were (58.1 ±1.2)%and (14.5 ±1.4)%under these conditions .The nanop-articles were spherical and uniform under TEM .The average particle size was 330.3 nm, and the nanoparticles distributed evenly (PDI was 0.311), zeta potential was 39.2 mV.Conclusions Thin film hydration method is suitable for the prepa-ration of Cur-GA-PEI-PLGA nanoparticles .The optimum preparation conditions are as follows:the hydration temperature is 60 ℃, the hydration time is 3 h, and the drug addition is 7 mg.
