CAJ | 학술논문

目的：观察淫羊藿苷及脱水淫羊藿素对人牙髓细胞增殖和成矿能力的影响。方法将人牙髓细胞随机分为观察A组、观察B组和对照组。观察A、B组分别加入不同浓度淫羊藿苷、脱水淫羊藿素，对照组不予处理。采用CCK-8法检测各组增殖能力（以OD450表示），Real-time PCR法检测骨钙素（OCN）和牙本质涎磷蛋白（DSPP） mRNA，碱性磷酸酶染色和茜素红染色观察各组成矿能力。结果观察A组加入0．1、1、10μmol／L淫羊藿苷后OD450分别为1．86±0．08、1．96±0．07、1．82±0．04，观察B组加入0．1、1、10μmol／L脱水淫羊藿素后OD450分别为1．90±0．07、1．93±0．09、1．88±0．02，对照组为1．76±0．10；观察A、B组均高于对照组，P均＜0．05；观察A、B组比较，P均＞0．05。观察A、B组OCN、DSPP mRNA表达均高于对照组，P均＜0．05；观察A、B组比较，P均＞0．05。在成骨诱导剂存在情况下，观察A、B组和对照组的染色结果无明显差别。结论淫羊藿苷和脱水淫羊藿素均能提高人牙髓细胞的增殖和成矿能力，但成矿能力与成骨诱导剂相比作用较微弱。
목적：관찰음양곽감급탈수음양곽소대인아수세포증식화성광능력적영향。방법장인아수세포수궤분위관찰A조、관찰B조화대조조。관찰A、B조분별가입불동농도음양곽감、탈수음양곽소，대조조불여처리。채용CCK-8법검측각조증식능력（이OD450표시），Real-time PCR법검측골개소（OCN）화아본질연린단백（DSPP） mRNA，감성린산매염색화천소홍염색관찰각조성광능력。결과관찰A조가입0．1、1、10μmol／L음양곽감후OD450분별위1．86±0．08、1．96±0．07、1．82±0．04，관찰B조가입0．1、1、10μmol／L탈수음양곽소후OD450분별위1．90±0．07、1．93±0．09、1．88±0．02，대조조위1．76±0．10；관찰A、B조균고우대조조，P균＜0．05；관찰A、B조비교，P균＞0．05。관찰A、B조OCN、DSPP mRNA표체균고우대조조，P균＜0．05；관찰A、B조비교，P균＞0．05。재성골유도제존재정황하，관찰A、B조화대조조적염색결과무명현차별。결론음양곽감화탈수음양곽소균능제고인아수세포적증식화성광능력，단성광능력여성골유도제상비작용교미약。
Objective To investigate the effects of icariin and anhydroicaritin on the proliferation and mineralization of human dental pulp cells (DPCs).Methods DPCs were divided into the observation groups A , B and the control group. Observation groups A and B were treated with icariin or anhydroicaritin at different concentrations , respectively;the control group was not treated .Cell proliferation was determined by CCK-8 assay ( OD450 represents the absorbance value at 450 nm wavelength).The real-time PCR was used to detect the mRNA expression of mineralization-related gene osteocalcin (OCN) and dentinogensis-specific gene dentin sialophosphoprotein (DSPP).Alkaline phosphatase (ALP) histochemical staining and alizarin red staining were used to evaluate the effects of icariin and anhydroicaritin on the mineralization capaci -ty of DPCs.Results After the cells were treated with 0.1, 1 and 10 μmol/L icariin, the OD450 values in the observation group A were 1.86 ±0.08, 1.96 ±0.07 and 1.82 ±0.04, respectively.After the cells were treated with 0.1, 1,10μmol/L anhydroicaritin, the OD450 values in the observation group B were 1.90 ±0.07, 1.93 ±0.09 and 1.88 ±0.02, re-spectively.Meanwhile, the OD450 value in the control group was 1.76 ±0.10.The OD450 values in the observation groups A and B were higher than that in the control group (all P<0.05).However, there was no significant difference between ob-servation groups A and B (all P>0.05).Similarly, OCN and DSPP mRNA expression in the observation groups A and B was higher than that in the control group (all P<0.05), and no significant difference was found between observation groups A and B (all P>0.05).There was no significant difference between observation groups A , B and the control group under osteoinductive condition .Conclusion Icariin and anhydroicaritin may promote proliferation and mineralization abili-ties of DPCs.However, compared with osteogenesis supplement , icariin and anhydroicaritin have a slight osteogenic effect on DPCs.