天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2015年
4期
379-382
,共4页
人乳头瘤病毒16%感染%宫颈肿瘤%问卷调查%危险因素%焦磷酸测序技术
人乳頭瘤病毒16%感染%宮頸腫瘤%問捲調查%危險因素%焦燐痠測序技術
인유두류병독16%감염%궁경종류%문권조사%위험인소%초린산측서기술
human papillomavirus 16%infection%uterine cervical neoplasms%questionnaires%risk factors%pyrosequencing
目的：检测天津市市区健康筛查女性中人乳头瘤病毒(HPV)16型感染率，分析HPV16感染的危险因素。方法通过问卷调查方式对2000例天津市市区健康普查女性进行相关因素调查，同时收集入组女性的宫颈脱落细胞。通过巢式PCR扩增和焦磷酸测序技术进行HPV型别检测，采用χ2检验和多因素Logistic回归分析HPV16感染的高危因素。结果2000例筛查标本中，HPV阳性271例（13.55%），其中前3位为HPV16型阳性占39.5%（107/271），HPV58型阳性占15.13%（41/271），HPV18型阳性占9.59%（26/271）。HPV16感染率吸烟者高于不吸烟者，首次性交年龄≤25岁者高于＞25岁者，性伴侣数≥2者高于＜2者，怀孕次数≥2者高于＜2者，流产次数≥3者高于＜3者，避孕方式为非避孕套者高于避孕套者（均P＜0.05）；HPV16感染率在不同年龄、饮酒史、教育水平、以前妇科体检等因素间差异无统计学意义。吸烟史和首次性交年龄≤25岁为HPV16感染的独立危险因素。结论天津市市区健康普查妇女HPV感染以HPV16型最常见。避免高危因素的暴露，制止女性过早发生性行为，同时改变不良生活习惯是预防HPV16感染及降低宫颈癌发生的有效措施。
目的：檢測天津市市區健康篩查女性中人乳頭瘤病毒(HPV)16型感染率，分析HPV16感染的危險因素。方法通過問捲調查方式對2000例天津市市區健康普查女性進行相關因素調查，同時收集入組女性的宮頸脫落細胞。通過巢式PCR擴增和焦燐痠測序技術進行HPV型彆檢測，採用χ2檢驗和多因素Logistic迴歸分析HPV16感染的高危因素。結果2000例篩查標本中，HPV暘性271例（13.55%），其中前3位為HPV16型暘性佔39.5%（107/271），HPV58型暘性佔15.13%（41/271），HPV18型暘性佔9.59%（26/271）。HPV16感染率吸煙者高于不吸煙者，首次性交年齡≤25歲者高于＞25歲者，性伴侶數≥2者高于＜2者，懷孕次數≥2者高于＜2者，流產次數≥3者高于＜3者，避孕方式為非避孕套者高于避孕套者（均P＜0.05）；HPV16感染率在不同年齡、飲酒史、教育水平、以前婦科體檢等因素間差異無統計學意義。吸煙史和首次性交年齡≤25歲為HPV16感染的獨立危險因素。結論天津市市區健康普查婦女HPV感染以HPV16型最常見。避免高危因素的暴露，製止女性過早髮生性行為，同時改變不良生活習慣是預防HPV16感染及降低宮頸癌髮生的有效措施。
목적：검측천진시시구건강사사녀성중인유두류병독(HPV)16형감염솔，분석HPV16감염적위험인소。방법통과문권조사방식대2000례천진시시구건강보사녀성진행상관인소조사，동시수집입조녀성적궁경탈락세포。통과소식PCR확증화초린산측서기술진행HPV형별검측，채용χ2검험화다인소Logistic회귀분석HPV16감염적고위인소。결과2000례사사표본중，HPV양성271례（13.55%），기중전3위위HPV16형양성점39.5%（107/271），HPV58형양성점15.13%（41/271），HPV18형양성점9.59%（26/271）。HPV16감염솔흡연자고우불흡연자，수차성교년령≤25세자고우＞25세자，성반려수≥2자고우＜2자，부잉차수≥2자고우＜2자，유산차수≥3자고우＜3자，피잉방식위비피잉투자고우피잉투자（균P＜0.05）；HPV16감염솔재불동년령、음주사、교육수평、이전부과체검등인소간차이무통계학의의。흡연사화수차성교년령≤25세위HPV16감염적독립위험인소。결론천진시시구건강보사부녀HPV감염이HPV16형최상견。피면고위인소적폭로，제지녀성과조발생성행위，동시개변불량생활습관시예방HPV16감염급강저궁경암발생적유효조시。
Objective To investigate the incidence and risk factors of human papillomavirus type 16 (HPV16) infec?tion among women through health screenings in Tianjin urban area. Methods A total of 2 000 women from Tianjin urban area were recruited for this study, and potential risk factors were investigated by questionnaire as well as by collecting cervi?cal exfoliated cells from the participants. HPV type was examined using polymerase chain reaction and pyrosequencing tech?nology, then the risk factors of HPV16 infection were examined by chi square test and the multiple factor logistic regression analysis. Results Among those 2 000 women who received health screenings, a total of 271 cases were HPV positive (13.55%). HPV16 accounts for the most common human papillomavirus types (39.5%, 107/271) while HPV58 account for 15.13%(41/271)and HPV18 account for 9.59% (26/271). Factors related to HPV16 infection were investigated include smoking history, initial age of sex (>25 years old or not) , number of pregnancies (≥2 or not), number of sexual partners (≥2 or not) and contraception measures (condom or other ways) with χ2=6.268,8.540,4.951,7.123,6.053,5.019, respectively. (P < 0.05). Age, alcohol intake, education level and previous gynecologial examination did not differ significantly among HPV16 positive patients compared to those with negative HPV. Multiple factor Logistic regression model analysis showed that the independent factors were smoking history and initial age for sex(≤25 years old)(P<0.05). Conclusion HPV16 is the most common type of cervical infection among women in Tianjin urban area. Changing inappropriate sexual behavior and smoking cessation are effective measures to prevent HPV16 infection and reduce incidence of cervical cancer.
