中国药理学与毒理学杂志
中國藥理學與毒理學雜誌
중국약이학여독이학잡지
CHINESE JOURNAL OF PHARMACOLOGY AND TOXICOLOGY
2015年
2期
208-212
,共5页
黄立双%刘伟丽%弓景波%杲修杰%赵云%马婧%谢方%张涛%田凯琦%姚林%钱令嘉
黃立雙%劉偉麗%弓景波%杲脩傑%趙雲%馬婧%謝方%張濤%田凱琦%姚林%錢令嘉
황립쌍%류위려%궁경파%고수걸%조운%마청%사방%장도%전개기%요림%전령가
血管重塑%高血压%多沙唑嗪%美托洛尔
血管重塑%高血壓%多沙唑嗪%美託洛爾
혈관중소%고혈압%다사서진%미탁락이
vascular remodeling%hypertension%doxazosin%metroprolol
目的:观察多沙唑嗪及美托洛尔对腹主动脉缩窄(AAC)诱导的大鼠血管重塑的影响。方法采用 AAC 法成功建立血管重塑大鼠模型。2周后每天 ig 给予多沙唑嗪10 mg??kg-1或美托洛尔20 mg??kg-1,连续6周。采用颈总动脉插管测量大鼠的平均动脉压;采用 HE 染色和图像分析仪检测大鼠主动脉中膜厚度、中膜面积和中膜厚度与管腔内径比值;Masson 染色检测纤维化;Western 蛋白印迹法检测胶原蛋白和纤连蛋白表达。结果与假手术组血压(17.6±0.5) kPa 相比,模型组大鼠血压(23.3±0.7) kPa 显著升高(P<0.05),给予多沙唑嗪〔(20.5±0.7)kPa〕或美托洛尔〔(19.0±0.4) kPa〕均可有效降低血压(P<0.05)。 HE染色结合图像分析仪分析发现,模型组大鼠血管中膜厚度、中膜面积及中膜厚度与血管内径比值较假手术组分别升高39.5%,46.4%和27.0%(P<0.05),给予多沙唑嗪或美托洛尔使血管的中膜厚度分别下降16.0%和26.1%(P<0.05),中膜面积分别下降22.8%和29.1%(P<0.05),中膜厚度与血管内径比值分别下降17.0%和26.0%(P<0.05)。 Masson 染色结果显示,与假手术组相比,模型组大鼠血管组织胶原蛋白的沉积增多,血管出现纤维化;而给予多沙唑嗪或美托洛尔可以缓解高血压诱导的纤维化发生。 Western 蛋白印迹结果显示,模型组大鼠血管组织中胶原蛋白和纤连蛋白的表达明显增加(P<0.05),而多沙唑嗪组和美托洛尔组较模型组胶原蛋白和纤连蛋白表达显著下降(P<0.05)。结论多沙唑嗪和美托洛尔可能通过抑制去甲肾上腺素与其特异性受体结合,从而选择性地阻断去甲肾上腺素对血管的诱导作用,逆转高血压诱导的血管重塑的发生。
目的:觀察多沙唑嗪及美託洛爾對腹主動脈縮窄(AAC)誘導的大鼠血管重塑的影響。方法採用 AAC 法成功建立血管重塑大鼠模型。2週後每天 ig 給予多沙唑嗪10 mg??kg-1或美託洛爾20 mg??kg-1,連續6週。採用頸總動脈插管測量大鼠的平均動脈壓;採用 HE 染色和圖像分析儀檢測大鼠主動脈中膜厚度、中膜麵積和中膜厚度與管腔內徑比值;Masson 染色檢測纖維化;Western 蛋白印跡法檢測膠原蛋白和纖連蛋白錶達。結果與假手術組血壓(17.6±0.5) kPa 相比,模型組大鼠血壓(23.3±0.7) kPa 顯著升高(P<0.05),給予多沙唑嗪〔(20.5±0.7)kPa〕或美託洛爾〔(19.0±0.4) kPa〕均可有效降低血壓(P<0.05)。 HE染色結閤圖像分析儀分析髮現,模型組大鼠血管中膜厚度、中膜麵積及中膜厚度與血管內徑比值較假手術組分彆升高39.5%,46.4%和27.0%(P<0.05),給予多沙唑嗪或美託洛爾使血管的中膜厚度分彆下降16.0%和26.1%(P<0.05),中膜麵積分彆下降22.8%和29.1%(P<0.05),中膜厚度與血管內徑比值分彆下降17.0%和26.0%(P<0.05)。 Masson 染色結果顯示,與假手術組相比,模型組大鼠血管組織膠原蛋白的沉積增多,血管齣現纖維化;而給予多沙唑嗪或美託洛爾可以緩解高血壓誘導的纖維化髮生。 Western 蛋白印跡結果顯示,模型組大鼠血管組織中膠原蛋白和纖連蛋白的錶達明顯增加(P<0.05),而多沙唑嗪組和美託洛爾組較模型組膠原蛋白和纖連蛋白錶達顯著下降(P<0.05)。結論多沙唑嗪和美託洛爾可能通過抑製去甲腎上腺素與其特異性受體結閤,從而選擇性地阻斷去甲腎上腺素對血管的誘導作用,逆轉高血壓誘導的血管重塑的髮生。
목적:관찰다사서진급미탁락이대복주동맥축착(AAC)유도적대서혈관중소적영향。방법채용 AAC 법성공건립혈관중소대서모형。2주후매천 ig 급여다사서진10 mg??kg-1혹미탁락이20 mg??kg-1,련속6주。채용경총동맥삽관측량대서적평균동맥압;채용 HE 염색화도상분석의검측대서주동맥중막후도、중막면적화중막후도여관강내경비치;Masson 염색검측섬유화;Western 단백인적법검측효원단백화섬련단백표체。결과여가수술조혈압(17.6±0.5) kPa 상비,모형조대서혈압(23.3±0.7) kPa 현저승고(P<0.05),급여다사서진〔(20.5±0.7)kPa〕혹미탁락이〔(19.0±0.4) kPa〕균가유효강저혈압(P<0.05)。 HE염색결합도상분석의분석발현,모형조대서혈관중막후도、중막면적급중막후도여혈관내경비치교가수술조분별승고39.5%,46.4%화27.0%(P<0.05),급여다사서진혹미탁락이사혈관적중막후도분별하강16.0%화26.1%(P<0.05),중막면적분별하강22.8%화29.1%(P<0.05),중막후도여혈관내경비치분별하강17.0%화26.0%(P<0.05)。 Masson 염색결과현시,여가수술조상비,모형조대서혈관조직효원단백적침적증다,혈관출현섬유화;이급여다사서진혹미탁락이가이완해고혈압유도적섬유화발생。 Western 단백인적결과현시,모형조대서혈관조직중효원단백화섬련단백적표체명현증가(P<0.05),이다사서진조화미탁락이조교모형조효원단백화섬련단백표체현저하강(P<0.05)。결론다사서진화미탁락이가능통과억제거갑신상선소여기특이성수체결합,종이선택성지조단거갑신상선소대혈관적유도작용,역전고혈압유도적혈관중소적발생。
OBJECTIVE To investigate the effect of doxazosin(DOX) and metoprolol( MET) on vascular remodeling in rats with abdominal aorta coarctation (AAC). METHODS An animal model was established by AAC. Two weeks later, the rats were treated with DOX (10 mg.kg-1 per day) or MET (20 mg.kg-1 per day) for six weeks. Blood pressure was measured using carotid artery intubation with a MP150 polygraph. The media thickness, wall cross-sectional area and thickness / internal diameter ratio were calculated by morphometry. Vascular fibrosis was evaluated by Masson′s trichrome staining. The collagen and fibronectin expression in vascules was measured by Western blotting. RESULTS Compared with the sham group 〔(17.6±0.5)kPa〕, the mean arterial blood pressure in the model group〔(23.3±0.7)kPa〕 was significantly increased(P<0.05), but was lowered by DOX 〔(20.5±0.7)kPa〕 and MET 〔(19.0±0.4) kPa〕 (P<0.05). Moreover, HE staining showed that tunica media thickness, artery vessel area and thickness / inner diameter in the model group were increased by 39.5%, 46.4% and 27.0%(P<0.05), respectively. The tunica media thickness was decreased by 16.0% and 26.1%( P<0.05), respectively, the artery vessel area by 22.8% and 26.1%(P<0.05), respectively, and the thick-ness / inner diameter by 17.0% and 26.0%( P<0.05) when the rats were treated with DOX and MET. Masson staining showed that the collagen accumulation in vascules increased, suggesting that AAC induced fibrosis. Meanwhile, vascular fibrosis induced by AAC was also reduced by MET or DOX. Western blotting also proved that the increase of collagen and fibronectin induced by AAC could be attenuated by DOX and MET(P<0.05). CONCLUSION DOX and MET are effective in suppressing the role of norepi-nephrine in vassels, which can attenuate AAC-induced vassels remodeling by preventing the binding between norepinephrine and adrenoceptors.