CAJ | 학술논문

目的：研究高效液相色谱—串联质谱法（ LC-MS／MS）法测定人血浆中双氯芬酸浓度的不确定度评定方法。方法对双氯芬酸浓度测定全过程进行分析，包括测定精密度、称量、标准溶液的配制、蛋白沉淀过程、标准曲线拟合等进行分析评定，最后根据各分量计算出合成不确定度并进行了扩展。结果置信概率为95％时，人血浆中2．0、50．0、1600μg· L－1浓度双氯芬酸的扩展不确定度分别为（1．97±1．482）、（46．97±3．72）、（1494±87．2）μg· L－1。结论该方法不确定度主要来自标准曲线的拟合过程，该法适用于评定LC-MS／MS法测定血浆中双氯芬酸的不确定度研究。
목적：연구고효액상색보—천련질보법（ LC-MS／MS）법측정인혈장중쌍록분산농도적불학정도평정방법。방법대쌍록분산농도측정전과정진행분석，포괄측정정밀도、칭량、표준용액적배제、단백침정과정、표준곡선의합등진행분석평정，최후근거각분량계산출합성불학정도병진행료확전。결과치신개솔위95％시，인혈장중2．0、50．0、1600μg· L－1농도쌍록분산적확전불학정도분별위（1．97±1．482）、（46．97±3．72）、（1494±87．2）μg· L－1。결론해방법불학정도주요래자표준곡선적의합과정，해법괄용우평정LC-MS／MS법측정혈장중쌍록분산적불학정도연구。
Objective To develop a method to evaluate the uncertainty in the determination of diclofenac in human plasma by LC-MS/MS.Methods The uncertainty sources were obtained from the determination of the whole procedure,including repeatability,weighing, solution preparation,protein precipitation process,the apparatus and calibration fitting.The combined uncertainty and expanded uncer-tainty were calculated accordingly.Results The expanded uncertainty for 2.0,50.0,1 600μg· L-1 level of diclofenac was (1.97 ± 1.482)、(46.97 ±3.72),and(1 494 ±87.2)μg· L-1 respectively (P=95%).Conclusion The uncertainty is mainly caused by calibration curve.This method is suitable for the uncertainty evaluation in human plasma by LC-MS/MS.