CAJ | 학술논문

目的：观察氧化槐果碱（OSC）对人结肠腺癌细胞株 SW480的增殖抑制及诱导凋亡的作用，探讨其作用机制。方法体外培养 SW480细胞，使用 OSC（作用浓度分别为0.5～2 mg／mL）和阳性对照药5－氟尿嘧啶（作用浓度10μg／mL）作用于细胞12～48 h；采用 MTT 法测定 SW480细胞增殖情况，荧光双染观察细胞形态变化，计数凋亡细胞所占比例，流式细胞仪分析细胞周期变化及细胞凋亡率，Real －time PCR 检测凋亡基因 Caspase －3和 Bcl －2的表达。结果MTT 结果显示，各浓度 OSC 对体外培养的 SW480细胞增殖具有明显抑制作用，该作用呈一定的时间和剂量依赖性；OSC 作用后细胞的体积变小变圆，核变形、皱缩，凋亡细胞所占比例显著增加；流式细胞仪分析结果表明，随着 OSC 作用时间的延长，G0／G1期细胞比例增加，同时 S 期细胞比例也相应增加；凋亡基因 Caspase －3 mRNA 表达上调，Bcl －2 mRNA 表达下调。结论OSC 可抑制体外培养的 SW480细胞生长增殖，其作用机制与阻滞细胞周期进程、诱导细胞凋亡有关。
목적：관찰양화괴과감（OSC）대인결장선암세포주 SW480적증식억제급유도조망적작용，탐토기작용궤제。방법체외배양 SW480세포，사용 OSC（작용농도분별위0.5～2 mg／mL）화양성대조약5－불뇨밀정（작용농도10μg／mL）작용우세포12～48 h；채용 MTT 법측정 SW480세포증식정황，형광쌍염관찰세포형태변화，계수조망세포소점비례，류식세포의분석세포주기변화급세포조망솔，Real －time PCR 검측조망기인 Caspase －3화 Bcl －2적표체。결과MTT 결과현시，각농도 OSC 대체외배양적 SW480세포증식구유명현억제작용，해작용정일정적시간화제량의뢰성；OSC 작용후세포적체적변소변원，핵변형、추축，조망세포소점비례현저증가；류식세포의분석결과표명，수착 OSC 작용시간적연장，G0／G1기세포비례증가，동시 S 기세포비례야상응증가；조망기인 Caspase －3 mRNA 표체상조，Bcl －2 mRNA 표체하조。결론OSC 가억제체외배양적 SW480세포생장증식，기작용궤제여조체세포주기진정、유도세포조망유관。
Objective To investigate the inhibition of oxysophocarpine (OSC) on proliferation of human colon cancer cell line SW480 and induction on apoptosis , and to explore its mechanism.Methods SW480 cells were treated with OSC (0.5 -2 mg/mL) and the positive control drug 5 -fluorouracil (10 μg/mL) for 12 -48 h.MTT assay was used to detect the proliferation of SW480 cells; fluorescence double staining was used to observe the changes in cell mor -phology and the ratio of apoptotic cells ; flow cytometry was used to demonstrate the presence of apoptosis ; and real -time PCR detection was used to demonstrate changes in gene Caspase -3 and Bcl -2 expression.Results The results of MTT showed that OSC significantly inhibited the growth of SW 480 cells in dose -and time -dependent manners, and morpho-logical characteristics of apoptosis were also observed .The percentages of the G 0 /G1 phase and the S phase cells were in-creased after treated by OSC.Apoptosis and G0 /G1 arrest were induced by OCS in SW480 cells.Apoptotic gene Caspase -3 mRNA was up -regulated, while Bcl -2 mRNA down -regulated.Conclusion OSC inhibits the proliferation of SW480 cells in vitro, involving arrest of cell cycle progression and apoptosis .