CAJ | 학술논문

目的探讨大鼠幽门切除+胃空肠Roux-en-Y吻合术后胃空肠吻合口再生的Cajal间质细胞(ICC)对吻合口区域慢波传导和胃排空功能的影响.方法 20只SD雄性大鼠随机均分为实验组和对照组,实验组行幽门切除+胃空肠Roux-en-Y吻合术,胃空肠吻合口两侧3 mm处浆膜下各植入铂金微电极1枚,对照组仅行幽门切断再吻合术,幽门上下各植入铂金微电极1枚.术后16周进行以下实验:放射性核素闪烁显像法评估胃液体排空功能;记录大鼠胃空肠吻合口和幽门两侧慢波;电镜观察胃空肠吻合口及其邻近区域组织的ICC再生情况.组间结果比较采用独立样本t检验.结果实验组大鼠胃液体半排空时间为(23.5 ±4.5) min,对照组为(10.2±2.3) min,两组差异有统计学意义(t =7.978,P=0.000).胃空肠吻合口两侧生物电活动紊乱,空肠慢波通过吻合口逆行向胃内传导.胃空肠吻合口及其邻近区域组织的ICC再生并相互连接形成网络状结构.结论大鼠幽门切除+胃空肠Roux-en-Y吻合术后胃空肠吻合口及其邻近区域组织的ICC再生并相互连接形成网络状结构,以此异常结构为基础可能造成局部电活动紊乱使胃液体排空明显延迟.
목적 탐토대서유문절제+위공장Roux-en-Y문합술후위공장문합구재생적Cajal간질세포(ICC)대문합구구역만파전도화위배공공능적영향.방법 20지SD웅성대서수궤균분위실험조화대조조,실험조행유문절제+위공장Roux-en-Y문합술,위공장문합구량측3 mm처장막하각식입박금미전겁1매,대조조부행유문절단재문합술,유문상하각식입박금미전겁1매.술후16주진행이하실험:방사성핵소섬삭현상법평고위액체배공공능;기록대서위공장문합구화유문량측만파;전경관찰위공장문합구급기린근구역조직적ICC재생정황.조간결과비교채용독립양본t검험.결과 실험조대서위액체반배공시간위(23.5 ±4.5) min,대조조위(10.2±2.3) min,량조차이유통계학의의(t =7.978,P=0.000).위공장문합구량측생물전활동문란,공장만파통과문합구역행향위내전도.위공장문합구급기린근구역조직적ICC재생병상호련접형성망락상결구.결론 대서유문절제+위공장Roux-en-Y문합술후위공장문합구급기린근구역조직적ICC재생병상호련접형성망락상결구,이차이상결구위기출가능조성국부전활동문란사위액체배공명현연지.
Objective To investigate the impact of the regeneration of interstitial cell of Cajal (ICC) on the conduction of slow wave and gastric emptying in rats undergoing Roux-en-Y gastrojejunostomy.Methods Twenty male SD rats were randomly divided into experimental group and control group.The experimental group consisted of ten rats undergoing Roux-en-Y gastrojejunostomy.The control group only underwent pyloric transection and anastomosis.Gastric scintigraphy was performed in the two groups respectively to measure the half time of gastric emptying (t1/2) at sixteen weeks after the surgical manipulations,and then the myoelectrical activities near the gastrojejunal anastomosis were recorded.The study also observed the regeneration of ICC by the electron microscopy.The data of the 2 groups was compared by t test.Results In the sixteenth postoperative week,the t1/2 was (23.5 ± 4.5) minutes for rats in the Roux-en-Y group and (10.2 ± 2.3) minutes for those in the control group,indicating delayed gastric emptying in the Roux-en-Y group (t =7.978,P =0.000),accompanied with the abnormal myoelectrical activities near the gastrojejunal anastomosis.The morphological detection showed that ICC near the gastrojejunal anastomosis regenerated and reconstructed their network in the rats of the experimental group.Conclusion The abnormal myoelectrical activities near the gastrojejunal anastomosis,basing on the regeneration and reconstruction of ICC,may make a significant delay on the gastric emptying.