河北北方学院学报（自然科学版）
河北北方學院學報（自然科學版）
하북북방학원학보（자연과학판）
JOURNAL OF HEBEI NORTH UNIVERSITY(NATURAL SCIENCE EDITION)
2015年
1期
71-74
,共4页
杜华阳%李秀娟%范婕%李玉珍
杜華暘%李秀娟%範婕%李玉珍
두화양%리수연%범첩%리옥진
Oct4%Sox2%食管鳞状细胞癌
Oct4%Sox2%食管鱗狀細胞癌
Oct4%Sox2%식관린상세포암
Oct4%Sox2%esophageal squamous cell carcinoma
目的：探讨 Oct4和 Sox2在食管鳞癌组织中的表达及二者之间的相关性。方法应用免疫组化 SP法和流式细胞术检测98例食管鳞癌标本及43例正常食管黏膜组织中 Oct4和 Sox2的表达情况，分析其与各临床病理特征的关系及二者之间的相关性。结果食管鳞癌组织 Oct4和 Sox2的 阳 性 表 达 率 均 高 于 正 常 组 织（P ＜0.05）；流式细胞术结果显示，癌组织中 Oct4和 Sox2的蛋白相对含量也明显高于正常组织（P ＜0.05）。随着分化程度的降低，Oct4和 Sox2的表达明显升高，即二者与食管癌组织的分化程度呈负相关（P ＜0.05），且 Oct4和 Sox2的表达与 TNM 分期、有无外膜浸润明显相关（P ＜0.05），而与患者年龄、性别无关。食管鳞癌组织中 Oct4和 Sox2表达呈正相关（r＝0.295，P ＝0.003）。结论Oct4和 Sox2在食管鳞癌的发生、发展过程中起重要作用，有望成为食管癌生物治疗的新靶点。
目的：探討 Oct4和 Sox2在食管鱗癌組織中的錶達及二者之間的相關性。方法應用免疫組化 SP法和流式細胞術檢測98例食管鱗癌標本及43例正常食管黏膜組織中 Oct4和 Sox2的錶達情況，分析其與各臨床病理特徵的關繫及二者之間的相關性。結果食管鱗癌組織 Oct4和 Sox2的 暘 性 錶 達 率 均 高 于 正 常 組 織（P ＜0.05）；流式細胞術結果顯示，癌組織中 Oct4和 Sox2的蛋白相對含量也明顯高于正常組織（P ＜0.05）。隨著分化程度的降低，Oct4和 Sox2的錶達明顯升高，即二者與食管癌組織的分化程度呈負相關（P ＜0.05），且 Oct4和 Sox2的錶達與 TNM 分期、有無外膜浸潤明顯相關（P ＜0.05），而與患者年齡、性彆無關。食管鱗癌組織中 Oct4和 Sox2錶達呈正相關（r＝0.295，P ＝0.003）。結論Oct4和 Sox2在食管鱗癌的髮生、髮展過程中起重要作用，有望成為食管癌生物治療的新靶點。
목적：탐토 Oct4화 Sox2재식관린암조직중적표체급이자지간적상관성。방법응용면역조화 SP법화류식세포술검측98례식관린암표본급43례정상식관점막조직중 Oct4화 Sox2적표체정황，분석기여각림상병리특정적관계급이자지간적상관성。결과식관린암조직 Oct4화 Sox2적 양 성 표 체 솔 균 고 우 정 상 조 직（P ＜0.05）；류식세포술결과현시，암조직중 Oct4화 Sox2적단백상대함량야명현고우정상조직（P ＜0.05）。수착분화정도적강저，Oct4화 Sox2적표체명현승고，즉이자여식관암조직적분화정도정부상관（P ＜0.05），차 Oct4화 Sox2적표체여 TNM 분기、유무외막침윤명현상관（P ＜0.05），이여환자년령、성별무관。식관린암조직중 Oct4화 Sox2표체정정상관（r＝0.295，P ＝0.003）。결론Oct4화 Sox2재식관린암적발생、발전과정중기중요작용，유망성위식관암생물치료적신파점。
Objective To study the expression of Oct4 and Sox2 proteins and their correlations in e-sophageal squamous cell carcinoma (ESCC).Methods Immunohistochemistry staining SP and folw cy-tometry (FCM)were used to detect the expression of Oct4 and Sox2 in 98 cases of ESCC and 43 normal mucous memebranes.Subsequently the relationship between postoperative pathological features was ana-lyzed with the expression of Oct4 and Sox2.Results The expressions of Oct4 and Sox2 in ESCC were ob-viously higher than that in normal esophageal (P <0.05).For the protein relative level detected by FCM, there was a significant deference between the ESCC and normal esophageal of the protein content of Oct4 and Sox2 (P <0.05).With the loss of differentiation degree,the expressions of Oct4 and Sox2 obviously rose in ESCC. The correlation is negative between Oct4 and Sox2 with the differentiation degree (P <0.05).The expressions of Oct4 and Sox2 were positively correlated to TNM stage and invasion of ad-ventitia (P <0.05).Oct4 expression in ESCC was positively correlated with that of Sox2 (r = 0.295, P =0.003).Conclusion Oct4 and Sox2 play crucial roles in occurrence and progression and are useful for the target therapy of ESCC.
