化学分析计量
化學分析計量
화학분석계량
CHEMICAL ANALYSIS AND METERAGE
2015年
2期
1-4,5
,共5页
陈伟珠%张怡评%方华%易瑞灶%朱芮琳%洪专
陳偉珠%張怡評%方華%易瑞竈%硃芮琳%洪專
진위주%장이평%방화%역서조%주예림%홍전
硫酸氨基葡萄糖%标准样品%均匀性%稳定性%定值%不确定度
硫痠氨基葡萄糖%標準樣品%均勻性%穩定性%定值%不確定度
류산안기포도당%표준양품%균균성%은정성%정치%불학정도
glucosamine sulfate%certified reference material%homogeneity%stability%certification%uncertainty
研制了硫酸氨基葡萄糖标准样品。以盐酸氨基葡萄糖为原料,制备高纯硫酸氨基葡萄糖,采用红外光谱(IR)、高分辨质谱和核磁共振谱(NMR)进行结构确证。样品分装成200瓶样品后,采用高效液相色谱–蒸发光散射法进行均匀性检验、稳定性检验和定值分析。从样品中随机抽取15瓶进行均匀性检验,结果表明在95%的置信区间范围内样品均匀性良好。按照25℃长期试验稳定性(12个月)进行稳定性考察,结果表明在考察期间内样品稳定性良好。标准样品经国内8家具有分析资质的实验室进行协同定值,硫酸氨基葡萄糖标准样品定值结果为99.84%,相对扩展不确定度为0.18%(k=1.96)。该标准样品达到国家标准样品的技术要求,可用于有关硫酸氨基葡萄糖的分析方法校正和质量控制。
研製瞭硫痠氨基葡萄糖標準樣品。以鹽痠氨基葡萄糖為原料,製備高純硫痠氨基葡萄糖,採用紅外光譜(IR)、高分辨質譜和覈磁共振譜(NMR)進行結構確證。樣品分裝成200瓶樣品後,採用高效液相色譜–蒸髮光散射法進行均勻性檢驗、穩定性檢驗和定值分析。從樣品中隨機抽取15瓶進行均勻性檢驗,結果錶明在95%的置信區間範圍內樣品均勻性良好。按照25℃長期試驗穩定性(12箇月)進行穩定性攷察,結果錶明在攷察期間內樣品穩定性良好。標準樣品經國內8傢具有分析資質的實驗室進行協同定值,硫痠氨基葡萄糖標準樣品定值結果為99.84%,相對擴展不確定度為0.18%(k=1.96)。該標準樣品達到國傢標準樣品的技術要求,可用于有關硫痠氨基葡萄糖的分析方法校正和質量控製。
연제료류산안기포도당표준양품。이염산안기포도당위원료,제비고순류산안기포도당,채용홍외광보(IR)、고분변질보화핵자공진보(NMR)진행결구학증。양품분장성200병양품후,채용고효액상색보–증발광산사법진행균균성검험、은정성검험화정치분석。종양품중수궤추취15병진행균균성검험,결과표명재95%적치신구간범위내양품균균성량호。안조25℃장기시험은정성(12개월)진행은정성고찰,결과표명재고찰기간내양품은정성량호。표준양품경국내8가구유분석자질적실험실진행협동정치,류산안기포도당표준양품정치결과위99.84%,상대확전불학정도위0.18%(k=1.96)。해표준양품체도국가표준양품적기술요구,가용우유관류산안기포도당적분석방법교정화질량공제。
Glucosamine sulfate certified reference material was developed. Glucosamine sulfate was made from the glucosamine hydrochloride. The structure of glucosamine sulfate certified reference material was comfirmed by infrared spectroscopy (IR), high resolution mass spectral (HRMS) and nuclear magnetic resonance (NMR). The sample was divided into 200 bottles, the homogeneity and stability testing and quantitative analysis were carried out by high performance liquid chromatography with evaporative light-scattering detector (HPLC–ELSD). According to analysis procedure of homogeneity, 15 bottles of sample were randomly taken from 200 bottles, and the resultswere validated byF-test statistical methods.The stability inspection was carried on the long-term(12 months), and the results indicated that the period for glucosamine sulfate of storage was 12 months at 25℃. A cooperative certification was conducted with 8 qualified laboratories. The certified purity value of the reference material of glucosamine sulfate was 99.84% with a relative expaned uncertainty of 0.18%(k=1.96). The reference material can conform to the technical requirement of the certified reference material. The material was intended for use in the method validation and quality control regarding glucosamine sulfate.