CAJ | 학술논문

研制一种对罗丹明B具有特异性识别性能的分子印迹固相萃取小柱。用沉淀聚合法制备罗丹明B分子印迹聚合物，通过静态平衡吸附实验及固相萃取实验表征其性能，并对市售辣椒样品中的罗丹明B进行测量。罗丹明B模板聚合物的吸附能力明显优于空白聚合物；印迹固相萃取小柱对罗丹明B标准溶液(0.05 mmol／L)一次性萃取率为98.24%，实际样品测量的回收率为90.0%～95.0%，测定结果的相对标准偏差为0.9%~1.7%(n=3)。罗丹明B分子印迹固相萃取小柱选择性好、萃取率高，可应用于食品、化妆品检测等相关领域。
연제일충대라단명B구유특이성식별성능적분자인적고상췌취소주。용침정취합법제비라단명B분자인적취합물，통과정태평형흡부실험급고상췌취실험표정기성능，병대시수랄초양품중적라단명B진행측량。라단명B모판취합물적흡부능력명현우우공백취합물；인적고상췌취소주대라단명B표준용액(0.05 mmol／L)일차성췌취솔위98.24%，실제양품측량적회수솔위90.0%～95.0%，측정결과적상대표준편차위0.9%~1.7%(n=3)。라단명B분자인적고상췌취소주선택성호、췌취솔고，가응용우식품、화장품검측등상관영역。
Solid phase extraction (SPE) column of Rhodamine B molecularly imprinted polymer (MIP) was prepared. Rhodamine B MIP was prepared by precipitation polymerization, whose absorption capability was characterized by equilibrium absorption experiment and SPE experiment. The colum was applied to the determination of Rhodamin B in pepper samples. The absorption capability of Rhodamine B MIP was significantly superior to that of NMIP. The extraction rate of MIP–SPE was 98.24% and the recoveries ranged from 90.0% to 95.0%, the relative deviation of detection results was 0.9%–1.7% (n=3). Rhodamine B MIP presented a good specific recognition to Rhodamine B. SPE column has good selectivity and high extraction rate, which can be applied in food and cosmetics testingfields.