CAJ | 학술논문

目的：研究半乳凝素-9（galectin-9）对胶质瘤细胞系 U251增殖和迁移能力的影响，并探讨其作用机制。方法利用实时定量 PCR 方法检测胶质瘤细胞系中 galectin-9的表达情况；加入不同浓度（1、4、8、16μg ／mL）外源galectin-9蛋白处理一定时间（12、24、36 h）后，CCK-8、Transwell 实验检测细胞增殖和迁移能力；另外，在galectin-9处理的同时加入其竞争性抑制剂乳糖，观察其对增殖、迁移作用的影响。结果4种胶质瘤细胞系均极低水平表达 galectin-9；8、16μg ／mL 浓度的 galectin-9处理组的增殖率较对照组明显降低（P ＜0．001），随着时间延长，细胞增殖率逐渐降低（P ＜0．01），添加乳糖组细胞增殖率相对于未加乳糖组增高（P ＜0．05）；外源性galectin-9刺激不同时间，穿至膜下的细胞数较对照组明显减少（P ＜0．01），添加乳糖组细胞数较未加乳糖组增多（P ＜0．05）。结论半乳凝素-9可明显抑制胶质瘤细胞的增殖，具有浓度和时间依赖性，并且能够有效抑制胶质瘤细胞的迁移。
목적：연구반유응소-9（galectin-9）대효질류세포계 U251증식화천이능력적영향，병탐토기작용궤제。방법이용실시정량 PCR 방법검측효질류세포계중 galectin-9적표체정황；가입불동농도（1、4、8、16μg ／mL）외원galectin-9단백처리일정시간（12、24、36 h）후，CCK-8、Transwell 실험검측세포증식화천이능력；령외，재galectin-9처리적동시가입기경쟁성억제제유당，관찰기대증식、천이작용적영향。결과4충효질류세포계균겁저수평표체 galectin-9；8、16μg ／mL 농도적 galectin-9처리조적증식솔교대조조명현강저（P ＜0．001），수착시간연장，세포증식솔축점강저（P ＜0．01），첨가유당조세포증식솔상대우미가유당조증고（P ＜0．05）；외원성galectin-9자격불동시간，천지막하적세포수교대조조명현감소（P ＜0．01），첨가유당조세포수교미가유당조증다（P ＜0．05）。결론반유응소-9가명현억제효질류세포적증식，구유농도화시간의뢰성，병차능구유효억제효질류세포적천이。
Objective To investigate the effects of exogenous galectin-9 on the proliferation and migration of glioma cells and its potential mechanism.Methods The expression of galectin-9 in glioma cells was detected with real-time quantitative polymerase chain reaction (PCR).After glioma cell line U251 was treated with different concentrations of exogenous galectin-9 (1,4,8,16 μg /mL),the changes of proliferation and migration were tested with cell count kit-8 (CCK-8)and transwell chambers respectively at different time (12,24,36 h).When the competitive inhibitor lac-tose was added,the changes in proliferation and migration were detected.Results The expression of galectin-9 in 4 glioma cell lines was low or barely detected.Cells treated with 8 and 16 μg /mL galectin-9 showed decreased cell prolif-eration rates,compared with control group (P <0.001).With time passing by,the cell proliferation rate decreased gradually (P <0.01).After lactose treatment,cell proliferation rate increased (P <0.05).Galectin-9 treatment signif-icantly reduced the numbers of cells penetrating the micropomus membrane (P <0.01 ),while lactose treatment in-creased the number of cells,compared with the untreated group (P <0.05).Conclusion Galectin-9 effectively inhib-its proliferation of glioma cells in a time-and dose-dependent manner,and it can also inhibit the migration of glioma cells.