CAJ | 학술논문

目的：探讨紫杉醇（PX）联合肿瘤坏死因子相关凋亡诱导配体（TRAIL）对胶质瘤 U251细胞凋亡作用的影响及其可能机制。方法分别采用改良 MTT 法和流式细胞术检测 PX 和 TRAIL 单独用药以及 TRAIL／PX 联合用药 U251细胞后细胞增殖和凋亡的情况，采用 Western bloting 法检测 PX 对 U251细胞 TRAIL 受体 DR4和 DR5表达的影响。结果TRAIL 和 PX 单独用药对 U251细胞增殖均具有抑制作用且呈浓度依赖性，TRAIL／PX 联合作用对 U251细胞生长抑制率和凋亡率均大于单独用药（P ＜0．05），TRAIL／PX 联合用药与单独用药相比可显著上调 DR4表达（P ＜0．05），DR5表达无明显变化。结论PX 可协同 TRAIL 上调 DR4的表达，进而提高胶质瘤细胞对 TRAIL 的敏感性，抑制细胞增殖和诱导细胞凋亡。
목적：탐토자삼순（PX）연합종류배사인자상관조망유도배체（TRAIL）대효질류 U251세포조망작용적영향급기가능궤제。방법분별채용개량 MTT 법화류식세포술검측 PX 화 TRAIL 단독용약이급 TRAIL／PX 연합용약 U251세포후세포증식화조망적정황，채용 Western bloting 법검측 PX 대 U251세포 TRAIL 수체 DR4화 DR5표체적영향。결과TRAIL 화 PX 단독용약대 U251세포증식균구유억제작용차정농도의뢰성，TRAIL／PX 연합작용대 U251세포생장억제솔화조망솔균대우단독용약（P ＜0．05），TRAIL／PX 연합용약여단독용약상비가현저상조 DR4표체（P ＜0．05），DR5표체무명현변화。결론PX 가협동 TRAIL 상조 DR4적표체，진이제고효질류세포대 TRAIL 적민감성，억제세포증식화유도세포조망。
Objective To explore the effects of paclitaxel (PX)in combination with tumor necrosis factor-related apop-tosis-inducing ligand (TRAIL)on glioma U251 cell apoptosis and its possible mechanism.Methods The proliferation and apoptosis of U251 cells treated by TRAIL/PX in combination or alone at different concentrations were detected with modified MTT and flow cytometry.Proteins of U251 cell TRAIL death receptor DR4 and DR5 were measured with Western blot assay.Results Both TRAIL and PX had inhibitory effect on U251 cell proliferation in a concentration-dependent manner,combined TRAIL/PX treatment had remarkably higher inhibitory effect on cell growth and apoptosis of U251 cells than either drug used lone (P <0.05).Furthermore,combined TRAIL/PX treatment showed a synergis-tic effect on U251 to upregulate the expression of DR4 (P <0.05),but had no effect on the expression of DR5. Conclusions PX can improve the sensitivity of glioma U251 cells to TRAIL by upregulation of DR4,and then inhibit glioma cell proliferation and induce apoptosis.