CAJ | 학술논문

从热激后耐热的牡丹品种‘凤丹白’中克隆到牡丹PseIF5A，全长480bp，与葡萄的同源基因具有较高的同源性，为热激诱导表达基因。将该基因转入大肠杆菌后，重组的菌株能诱导表达该基因的融合蛋白，并提高了对高温、低温、重金属、高盐、高Ph、氧化等非生物胁迫的抗性，表明牡丹PseIF5A能响应多种非生物胁迫。
종열격후내열적모단품충‘봉단백’중극륭도모단PseIF5A，전장480bp，여포도적동원기인구유교고적동원성，위열격유도표체기인。장해기인전입대장간균후，중조적균주능유도표체해기인적융합단백，병제고료대고온、저온、중금속、고염、고Ph、양화등비생물협박적항성，표명모단PseIF5A능향응다충비생물협박。
PseIF5A, a gene induced by heat stress, had been cloned from ‘Fengdanbai’, one heat-tolerance variety of paeonia suffructicosa and its ORF has 480bp, with hing homology comparison of nucleotide and amino acid sequence in VveIF5A from grape. Recombinant PseIF5A had detected in recombine E.coli BL21 by SDS-PAGE and western blot and its expression had made recombine E. coli increase the abiotic stresses, such as hing temperature, low temperature, heavy metal, salt, hing Ph and oxidation. So, this research result had indicated PseIF5A can response to Various abiotic stresses.