CAJ | 학술논문

目的：探讨乳牙和恒牙牙髓组织中 P2X3受体的差异及与炎症引发的牙齿疼痛的关系。方法：选择14个正常恒牙、10个正常乳牙及疼痛乳恒牙各12个的牙髓组织为实验对象，用基质金属蛋白酶－9（MMP－9）来显示牙髓的炎症状态；采用荧光定量 PCR 方法检测并比较人正常与疼痛乳恒牙牙髓组织中 P2X3受体 mRNA 的表达水平。结果：恒牙疼痛组与正常组中 P2X3受体 mRNA 的表达水平无明显差异（P ＞0．05），乳牙疼痛组中 P2X3受体的表达较正常组增强（P ＜0．05），而乳牙正常组与疼痛组中 P2X3受体的表达水平均分别高于恒牙正常组和疼痛组（P ＜0．05）。结论：P2X3受体可能参与乳牙牙髓炎疼痛的发生。
목적：탐토유아화항아아수조직중 P2X3수체적차이급여염증인발적아치동통적관계。방법：선택14개정상항아、10개정상유아급동통유항아각12개적아수조직위실험대상，용기질금속단백매－9（MMP－9）래현시아수적염증상태；채용형광정량 PCR 방법검측병비교인정상여동통유항아아수조직중 P2X3수체 mRNA 적표체수평。결과：항아동통조여정상조중 P2X3수체 mRNA 적표체수평무명현차이（P ＞0．05），유아동통조중 P2X3수체적표체교정상조증강（P ＜0．05），이유아정상조여동통조중 P2X3수체적표체수평균분별고우항아정상조화동통조（P ＜0．05）。결론：P2X3수체가능삼여유아아수염동통적발생。
Abstract] AIM:To investigate the relationship between dental pain and P2X3 receptor expression level. METHODS:14 normal permanent molars,10 normal primary teeth,12 pulpitis permanent molars and 12 pulpitis pri-mary teeth were included.An increase in metalloproteinase-9(MMP-9)was used to indicate pulpal inflammation in inflamed teeth.P2X3 receptor mRNA expression in tooth pulp was investigated using Real-Time fluorescence quantita-tive PCR.RESULTS:P2X3 receptor mRNA expression in normal and inflamed human permanent tooth pulp was simi-lar (P >0.05),whereas P2X3 receptor mRNA was up-regulated in inflamed primary dental pulp as compared with normal primary dental pulp (P <0.05).In addition,P2X3 receptor expression was greater in primary dental pulp than that in permanent dental pulp (P <0.05).CONCLUSION:P2X3 receptor may be related to primary tooth pulpitis pain.