CAJ | 학술논문

为了获得用于研制微生态制剂的乳酸杆菌菌株，从健康仔猪小肠中利用MRS固体培养基分离得到一株革兰氏阳性杆菌，通过培养特性、染色特征、菌落形态以及生化鉴定结果，初步判定该菌株为乳酸杆菌，再结合16S rDNA分子鉴定进一步确定该菌株为乳酸杆菌。牛津杯法体外抑菌试验结果显示，该菌对致病性大肠杆菌的抑菌效果较好，可以作为微生态制剂的保存菌种。
위료획득용우연제미생태제제적유산간균균주，종건강자저소장중이용MRS고체배양기분리득도일주혁란씨양성간균，통과배양특성、염색특정、균락형태이급생화감정결과，초보판정해균주위유산간균，재결합16S rDNA분자감정진일보학정해균주위유산간균。우진배법체외억균시험결과현시，해균대치병성대장간균적억균효과교호，가이작위미생태제제적보존균충。
In order to obtain Lactobacillus strains for the study of micro ecological preparation, we used the MRS solid medium to isolate a gram positive bacillus from small intestine of healthy piglets. Through the cultural characteristics, colony morphology and staining characteristics, biochemical identification results, we initially de-termined that the strain of Lactobacillus, combined with PCR to determine the strain of Lactobacillus analysis. The Oxford cup method in vitro bacteriostasis experiment results show that the lactobacillus effect on nosogenetic E.coli better and the Lactobacillus can be used as a preservation strains of probiotics.