CAJ | 학술논문

目的探讨在乙肝病毒X蛋白(HBx)诱导肝癌细胞上皮-间充质转化(EMT)过程中癌基因c-Src的作用.方法通过HBx基因转染诱导SMMC-7721肝癌细胞发生上皮-间充质转化,量化分析实验组和对照组细胞c-Src激酶的活化情况.抑制c-Src活性,通过实时PCR、蛋白印迹、免疫细胞化学等方法评价c-Src活化对上皮-间充质转化的影响.结果发生上皮-间充质转化的SMMC-7721细胞中c-Src活化显著增加.给予c-Src激酶抑制剂PP2后,细胞由梭形恢复原来的上皮表型,而给予安慰剂PP3细胞形态无明显变化.蛋白印迹、免疫细胞化学检测进一步证实,抑制c-Src活化能逆转HBx基因转染诱导的上皮-间充质转化,使细胞再次发生间叶-上皮样转化,即上皮标志物(E-cadherin、α-catenin)表达显著上调,间叶标志物(N-cadherin,Vimentin,Fibronectin)表达显著减少.结论 HBx蛋白诱导SMMC-7721肝癌细胞发生上皮-间充质转化时,c-Src活化发挥了关键作用.
목적 탐토재을간병독X단백(HBx)유도간암세포상피-간충질전화(EMT)과정중암기인c-Src적작용.방법 통과HBx기인전염유도SMMC-7721간암세포발생상피-간충질전화,양화분석실험조화대조조세포c-Src격매적활화정황.억제c-Src활성,통과실시PCR、단백인적、면역세포화학등방법평개c-Src활화대상피-간충질전화적영향.결과 발생상피-간충질전화적SMMC-7721세포중c-Src활화현저증가.급여c-Src격매억제제PP2후,세포유사형회복원래적상피표형,이급여안위제PP3세포형태무명현변화.단백인적、면역세포화학검측진일보증실,억제c-Src활화능역전HBx기인전염유도적상피-간충질전화,사세포재차발생간협-상피양전화,즉상피표지물(E-cadherin、α-catenin)표체현저상조,간협표지물(N-cadherin,Vimentin,Fibronectin)표체현저감소.결론 HBx단백유도SMMC-7721간암세포발생상피-간충질전화시,c-Src활화발휘료관건작용.
Objective To examine the role of c-Src activation in hepatitis B virus X (HBx) protein induced epithelial-mesenchymal transition (EMT) in liver cancer.Methods SMMC-7721 liver cancer cells were transfected with HBx gene to induce EMT and the activated c-Src expression was evaluated by Western blot.Both the morphological changes and the epithelial and mesenchymal markers expression (real-time PCR,western blot and immunocytochemistry) of HBx-transfected SMMC-7721 cell treated by c-Src kinase inhibitor PP2 and negative control PP3 were observed and compared,respectively.Results The activated c-Src expression in HBx gene transfected SMMC-7721 cells was significantly increased compared to that in mock transfected cells,c-Src kinase inhibitor PP2 could enable the HBx-transfected SMMC-7721 cells to transmit from spindle-like shape to original epithelial morphology.Western blot and immunocytochemistry confirmed that the expression of epithelial markers and mesenchymal markers almost returned to the levels of parental cells,indicating the mesenchymal-epithelial transition.Conclusions c-Src activation plays a key role in the process of EMT induced by HBx protein in SMMC-7721 cells.