山东大学学报（医学版）
山東大學學報（醫學版）
산동대학학보（의학판）
JOURNAL OF SHANDONG UNIVERSITY(HEALTH SCIENCES)
2015年
4期
37-42
,共6页
新生%低血糖%神经元%认知功能%大鼠,Wistar
新生%低血糖%神經元%認知功能%大鼠,Wistar
신생%저혈당%신경원%인지공능%대서,Wistar
Neonate%Hypoglycemia%Neurodegeneration%Cognitive function%Rat,Wistar
目的：探讨丙酮酸钠对新生鼠低血糖脑损伤的神经保护作用。方法36只新生 Wistar 大鼠随机分为胰岛素处理 P 组（INS-P 组，n ＝12）、胰岛素＋丙酮酸钠处理组（INS-PP 组，n ＝12）和对照组（n ＝12）。在出生后第2、4、6天分别采用皮下注射胰岛素（15 U／kg）诱导新生鼠低血糖；终止低血糖时 INS-P 组和 INS-PP 组分别皮下注射50％葡萄糖0．01 mL 和50％葡萄糖0．01 mL ＋丙酮酸钠500 mg ／kg；第3次低血糖处理后1天三组大鼠各取 6只取脑组织标本，并采用 Fluoro-Jade B（FJB）染色法进行死亡神经元的计数。低血糖损伤6周后，三组各 6只大鼠用Morris 水迷宫实验检测大鼠的空间学习和记忆能力。结果INS-PP 组与 INS-P 组相比，变性神经元计数为少，出生后6周 INS-PP 组大鼠的空间认知能力优于 INS-P 组，表现在 INS-PP 组大鼠找到平台的潜伏期短，在池中游泳距离短，在平台所在象限的逗留时间长，穿过平台位置的次数多。结论丙酮酸钠对胰岛素诱导的新生鼠低血糖脑损伤具有保护作用。丙酮酸钠给药可能是防止新生儿低血糖脑损伤的有效干预手段。
目的：探討丙酮痠鈉對新生鼠低血糖腦損傷的神經保護作用。方法36隻新生 Wistar 大鼠隨機分為胰島素處理 P 組（INS-P 組，n ＝12）、胰島素＋丙酮痠鈉處理組（INS-PP 組，n ＝12）和對照組（n ＝12）。在齣生後第2、4、6天分彆採用皮下註射胰島素（15 U／kg）誘導新生鼠低血糖；終止低血糖時 INS-P 組和 INS-PP 組分彆皮下註射50％葡萄糖0．01 mL 和50％葡萄糖0．01 mL ＋丙酮痠鈉500 mg ／kg；第3次低血糖處理後1天三組大鼠各取 6隻取腦組織標本，併採用 Fluoro-Jade B（FJB）染色法進行死亡神經元的計數。低血糖損傷6週後，三組各 6隻大鼠用Morris 水迷宮實驗檢測大鼠的空間學習和記憶能力。結果INS-PP 組與 INS-P 組相比，變性神經元計數為少，齣生後6週 INS-PP 組大鼠的空間認知能力優于 INS-P 組，錶現在 INS-PP 組大鼠找到平檯的潛伏期短，在池中遊泳距離短，在平檯所在象限的逗留時間長，穿過平檯位置的次數多。結論丙酮痠鈉對胰島素誘導的新生鼠低血糖腦損傷具有保護作用。丙酮痠鈉給藥可能是防止新生兒低血糖腦損傷的有效榦預手段。
목적：탐토병동산납대신생서저혈당뇌손상적신경보호작용。방법36지신생 Wistar 대서수궤분위이도소처리 P 조（INS-P 조，n ＝12）、이도소＋병동산납처리조（INS-PP 조，n ＝12）화대조조（n ＝12）。재출생후제2、4、6천분별채용피하주사이도소（15 U／kg）유도신생서저혈당；종지저혈당시 INS-P 조화 INS-PP 조분별피하주사50％포도당0．01 mL 화50％포도당0．01 mL ＋병동산납500 mg ／kg；제3차저혈당처리후1천삼조대서각취 6지취뇌조직표본，병채용 Fluoro-Jade B（FJB）염색법진행사망신경원적계수。저혈당손상6주후，삼조각 6지대서용Morris 수미궁실험검측대서적공간학습화기억능력。결과INS-PP 조여 INS-P 조상비，변성신경원계수위소，출생후6주 INS-PP 조대서적공간인지능력우우 INS-P 조，표현재 INS-PP 조대서조도평태적잠복기단，재지중유영거리단，재평태소재상한적두류시간장，천과평태위치적차수다。결론병동산납대이도소유도적신생서저혈당뇌손상구유보호작용。병동산납급약가능시방지신생인저혈당뇌손상적유효간예수단。
Objective To investigate the neuroprotective effect of sodium pyruvate on newborn Wistar rats suffered from repetitive and profound insulin-induced hypoglycemic brain injury.Methods A total of 36 newborn Wistar rats were randomly divided into three groups:insulin-treated P group (INS-P group,n =12),insulin and sodium pyruvate-treated group (INS-PP group,n =12),and control group (n =12).On postnatal day 2,4 and 6,insulin were injected hypodermicly into rats to induce hypoglycemia.After hypoglycemia lasting for 2.5 hours,dextrose was injected hypo-dermicly into rats in INS-P group,while dextrose was injected hypodermicly and sodium pyruvate was injected perito-neally into rats in INS-PP group.On 1 day after the third hypoglycemic insult,the neuronal degeneration in the rats′brains was examined by FJB staining.When the rats were 6 weeks old,their spatial learning and memory ability were detected with Morris’water maze experiment.Results Compared with INS-P group,the number of degenerated neu-rons in INS-PP group reduced and spatial learning and memory ability decreased.For the rats in INS-PP group,the spending time to find the platform was less,swimming distance was shorter,lingering time in the quadrant of platform was longer,and times to cross the platform were increased compared with those in INS-P group.Conclusion Sodium pyruvate can provide a neuroprotective effect on rats suffered from repetitive and profound neonatal hypoglycemia.Sodi-um pyruvate administration may be an effective intervention for patients with severe neonatal hypoglycemia to prevent brain injury.
