CAJ | 학술논문

目的：研究缺血性脑卒中大鼠缺血周边脑组织的经典 Wnt 信号通路活性及干预该通路活性对缺血周边血管新生的影响。方法随机将50只雄性 Wistar 大鼠分为4组：假手术组（Sham 组，n ＝10），脑缺血对照组（MCAO 组，n ＝20），氯化锂干预组（LiCl 组，n ＝10），DKK1干预组（DKK1组，n ＝10）。免疫印迹法检测经典Wnt 信号通路成分，FITC-dextran 检测血管密度，多普勒血流仪检测血流量。结果脑缺血1 d 后，与 Sham 组相比，MCAO 组缺血周边脑组织细胞核蛋白及细胞浆蛋白中β-catenin 表达水平显著下降（P ＜0．05），7 d 时明显恢复，14 d 时接近正常水平。与 MCAO 组相比，LiCl 组β-catenin 表达水平及 p-GSK-3β／GSK-3β比值提高（P ＜0．05），并且缺血周边血管密度及血流量增加（P ＜0．05）；DKK1组变化相反，β-catenin 表达水平及 p-GSK-3β／GSK-3β比值降低（P ＜0．05），血管密度及血流量下降（P ＜0．05）。结论经典 Wnt 信号通路参与调节缺血性脑卒中后缺血周边的血管新生。
목적：연구결혈성뇌졸중대서결혈주변뇌조직적경전 Wnt 신호통로활성급간예해통로활성대결혈주변혈관신생적영향。방법수궤장50지웅성 Wistar 대서분위4조：가수술조（Sham 조，n ＝10），뇌결혈대조조（MCAO 조，n ＝20），록화리간예조（LiCl 조，n ＝10），DKK1간예조（DKK1조，n ＝10）。면역인적법검측경전Wnt 신호통로성분，FITC-dextran 검측혈관밀도，다보륵혈류의검측혈류량。결과뇌결혈1 d 후，여 Sham 조상비，MCAO 조결혈주변뇌조직세포핵단백급세포장단백중β-catenin 표체수평현저하강（P ＜0．05），7 d 시명현회복，14 d 시접근정상수평。여 MCAO 조상비，LiCl 조β-catenin 표체수평급 p-GSK-3β／GSK-3β비치제고（P ＜0．05），병차결혈주변혈관밀도급혈류량증가（P ＜0．05）；DKK1조변화상반，β-catenin 표체수평급 p-GSK-3β／GSK-3β비치강저（P ＜0．05），혈관밀도급혈류량하강（P ＜0．05）。결론경전 Wnt 신호통로삼여조절결혈성뇌졸중후결혈주변적혈관신생。
Objective To investigate the involvement of canonical Wnt pathway in postischemic angiogenesis by using a rat model of ischemic stroke.Methods A total of 50 normal male Wistar rats were randomly divided into 4 groups:Sham group (n =10),cerebral ischemia group (MCAO group,n =20),LiCl treated group (LiCl group,n =10)and DKK1 treated group (DKK1 group,n =10).Western blotting was used to detect the expression of the elements of canonical Wnt pathway.FITC-dextran was used to detect the vessel volume,and Laser Doppler was used to measure the local cerebral blood flow in ischemic penumbral.Results The expressions of β-catenin in both the nuclear fraction and the cytoplasmic fraction in MCAO group decreased at 1 day post-cerebral ischemia,compared with Sham group (P <0.05).Nevertheless,the expressions of β-catenin in both the nuclear fraction and the cytoplasmic fraction at 7 day post-cerebral ischemia significantly increased (P <0.05),and reached normal level at 14 day post-cerebral ische-mia.Compared with MCAO group,the expressions of β-catenin in both the nuclear fraction and the cytoplasmic frac-tion LiCl group in were upregulated,accompanied with the elevated ratio of p-GSK-3β/GSK-3β,enhanced vessel vol-ume and local cerebral blood flow in ischemic penumbral(P <0.05).Compared with MCAO group,the expressions ofβ-catenin in DKK1 group in both the nuclear fraction and the cytoplasmic fraction were downregulated,accompanied with the decreased ratio of p-GSK-3β/GSK-3β,weakened vessel volume and local cerebral blood flow in ischemic penumbral(P <0.05).Conclusion The canonical Wnt pathway might be involved in postischemic angiogenesis.