CAJ | 학술논문

目的：探讨丁苯酞在大鼠脑创伤后海马神经组织中casepase-3表达及其在细胞凋亡中的作用。方法雄性Wistar大鼠120只随机分成3组，用Marmarou方法造成大鼠重型弥漫性颅脑创伤，采用免疫组织化学检测casepase-3蛋白表达情况，末端标记（ TUNEL）法观察大鼠脑内神经细胞凋亡动态变化。结果对照组海马区神经细胞casepase-3未见明显表达，创伤组海马区神经细胞casepase-3表达在伤后3 h开始升高，伤后3 d达高峰，伤后7 d下降明显。药物组海马区神经细胞casepase-3表达在伤后3 h开始升高，伤后3 d达高峰，高峰值低于创伤组（P＜0．01），伤后7 d下降明显。对照组海马区未见TUNEL阳性细胞，创伤组海马区TUNEL阳性细胞伤后6 h开始升高，3 d达高峰，伤后7 d下降。药物组海马区TUNEL阳性细胞伤后6 h开始升高，3 d达高峰，高峰值低于创伤组（P＜0．05），伤后7 d下降。 casepase-3表达与TUNEL阳性细胞明显相关（P＜0．01）。结论大鼠脑创伤后casepase-3的过度表达是影响大鼠脑创伤后神经细胞凋亡原因之一，丁苯酞可以抑制casepase-3活性表达，减少神经细胞凋亡，对神经组织起保护作用。
목적：탐토정분태재대서뇌창상후해마신경조직중casepase-3표체급기재세포조망중적작용。방법웅성Wistar대서120지수궤분성3조，용Marmarou방법조성대서중형미만성로뇌창상，채용면역조직화학검측casepase-3단백표체정황，말단표기（ TUNEL）법관찰대서뇌내신경세포조망동태변화。결과대조조해마구신경세포casepase-3미견명현표체，창상조해마구신경세포casepase-3표체재상후3 h개시승고，상후3 d체고봉，상후7 d하강명현。약물조해마구신경세포casepase-3표체재상후3 h개시승고，상후3 d체고봉，고봉치저우창상조（P＜0．01），상후7 d하강명현。대조조해마구미견TUNEL양성세포，창상조해마구TUNEL양성세포상후6 h개시승고，3 d체고봉，상후7 d하강。약물조해마구TUNEL양성세포상후6 h개시승고，3 d체고봉，고봉치저우창상조（P＜0．05），상후7 d하강。 casepase-3표체여TUNEL양성세포명현상관（P＜0．01）。결론대서뇌창상후casepase-3적과도표체시영향대서뇌창상후신경세포조망원인지일，정분태가이억제casepase-3활성표체，감소신경세포조망，대신경조직기보호작용。
Objective To explore the casepase-3 expression and role of casepase-3 in apoptosis following traumatic brain injury(TBI) in rat and the effect of dl-3-n-butylphthalide(NBP).Methods 120 male Wistar rats were randomly divided into 3 groups .According to Marmarou , severe closed brain injury was made .After that , immunohistochemical staining with Caspase-3 were performed of hippocampus CA1 region.TUNEL in situ was applied of the apoptosis cell in hippocampus CA 1 region.Double staining was to show the relation of apoptosis and Caspase -3.Results Caspase-3 in the trauma group began to increase at 3 h following injury , peaked at 3rd d and began to decline at 7 d. Caspase-3 in NBP treatment group was the same in each time , but in the peak , Caspase-3 was decreased in NBP treatment group compared with that in trauma group (P<0.01).At 3 h in trauma group , a little TUNEL positive cell appeared in hippocampus , peaked at 3rd d and began to decline at 7 d.TUNEL positive cell in NBP treatment group was the same in each time ,but in the peak , TUNEL positive cell was decreased in NBP treatment group compared with that in trauma group (P<0.05). The expression of casepase-3 was correlated with TUNEL positive cells ( P<0.01) .Conclusion NBP treatment in rats after TBI has certain neuroprotective effect , whose resistance roles in nerve cell apoptosis may be implemented by inhibiting excessive expressed caspase -3 caused by TBI .