CAJ | 학술논문

目的：探讨转化生长因子β（ TGF-β）和基质金属蛋白酶（ MMPs）在动脉钙化发生、发展中的作用。方法60只雄性SD大鼠随机分为实验组和对照组各30只。实验组连续3d皮下注射维生素D3注射液50万U／（ kg· d）制备动脉钙化模型，对照组同时注射等量葡萄糖注射液。两组同时间皮下注射同等剂量的不含维生素D3的5％葡萄糖注射液。采用Von Kossa 染色法观察动脉钙化程度；实时荧光定量PCR 法测定动脉组织 TGF-βmRNA和MMPs mRNA表达量。结果实验组动脉钙化程度、TGF-βmRNA和MMPs mRNA表达量均明显高于对照组（P均＜0．05）。结论 TGF-β和MMPs可促进动脉硬化的形成。
목적：탐토전화생장인자β（ TGF-β）화기질금속단백매（ MMPs）재동맥개화발생、발전중적작용。방법60지웅성SD대서수궤분위실험조화대조조각30지。실험조련속3d피하주사유생소D3주사액50만U／（ kg· d）제비동맥개화모형，대조조동시주사등량포도당주사액。량조동시간피하주사동등제량적불함유생소D3적5％포도당주사액。채용Von Kossa 염색법관찰동맥개화정도；실시형광정량PCR 법측정동맥조직 TGF-βmRNA화MMPs mRNA표체량。결과실험조동맥개화정도、TGF-βmRNA화MMPs mRNA표체량균명현고우대조조（P균＜0．05）。결론 TGF-β화MMPs가촉진동맥경화적형성。
Objective To explore the potential role of transforming growth factor-β( TGF-β) and matrix metallopro-teinases ( MMPs) in its occurrence and development of arterial calcification .Methods Sixty male SD rats were randomly divided into the control group and experimental group , with 30 rats in each group .Rats in the experimental group were in-jected vitamin D3 with a dose of 500 000 U/( kg· d) subcutaneously for 3 consecutive days , which was in order to induce the arterial calcification in rat models .The same dose of 5% glucose without vitamin D3 was injected into the rats of two groups subcutaneously .Using Von Kossa staining to observe the degree of tissue calcification of the rats , and using real-time fluorescent quantitative PCR to determinate the expression of mRNA of TGF-βand MMPs in the two groups .Results The degree of calcification and the mRNA expression of TGF-βand MMPs in the experimental group were significantly higher than those of the control group (all P<0.05).Conclusion The TGF-βand MMPs may contribute to the formation of arterial calcification .