肿瘤代谢与营养电子杂志
腫瘤代謝與營養電子雜誌
종류대사여영양전자잡지
Electronic Jourmal of Metabolism and Nutrition of Cancer
2015年
1期
42-45
,共4页
周蕊%卢宗亮%孔亚%夏万元%王佳佳%郭静%刘洁%杨剑%许红霞
週蕊%盧宗亮%孔亞%夏萬元%王佳佳%郭靜%劉潔%楊劍%許紅霞
주예%로종량%공아%하만원%왕가가%곽정%류길%양검%허홍하
桔梗皂苷D%前列腺癌%细胞周期
桔梗皂苷D%前列腺癌%細胞週期
길경조감D%전렬선암%세포주기
Platycodin D%Prostate cancer%Cell cycle
目的：研究来自桔梗的皂苷类单体化合物-桔梗皂苷D（platycodin D，PD）对前列腺癌细胞的杀伤效应及可能的机制。方法培养前列腺癌细胞株：PC3、DU145、LNCaP，四甲基偶唑氮（MTT）法检测PD对肿瘤细胞的存活率的影响；流式细胞仪检测PD阻滞细胞周期的能力；蛋白免疫印迹实验检测PD对周期相关蛋白表达的影响。结果PD杀伤前列腺癌细胞（PC3, DU145，LNCaP）的IC50值均小于30μM（PC3：11.17μM，DU145：26.13μM，LNCaP：24.6μM），随处理浓度增加，杀伤效应更为明显，呈明显剂量效应关系，其中以PC3细胞最为敏感。PD明显阻滞前列腺癌细胞周期，10μM PD、15μM PD将PC3细胞阻滞在G2/M期；而将DU145和LNCaP细胞阻滞在G0/G1期。PD影响PC3细胞的细胞周期相关蛋白：E2F1，CDK2，CDK4，CDK6，CyclinD1，Cdc2，CyclinB1蛋白表达量呈现下降趋势，与PD对细胞周期的阻滞作用相符合。结论PD能够杀伤前列腺癌细胞，阻滞细胞周期进展，从而抑制肿瘤的发展。
目的：研究來自桔梗的皂苷類單體化閤物-桔梗皂苷D（platycodin D，PD）對前列腺癌細胞的殺傷效應及可能的機製。方法培養前列腺癌細胞株：PC3、DU145、LNCaP，四甲基偶唑氮（MTT）法檢測PD對腫瘤細胞的存活率的影響；流式細胞儀檢測PD阻滯細胞週期的能力；蛋白免疫印跡實驗檢測PD對週期相關蛋白錶達的影響。結果PD殺傷前列腺癌細胞（PC3, DU145，LNCaP）的IC50值均小于30μM（PC3：11.17μM，DU145：26.13μM，LNCaP：24.6μM），隨處理濃度增加，殺傷效應更為明顯，呈明顯劑量效應關繫，其中以PC3細胞最為敏感。PD明顯阻滯前列腺癌細胞週期，10μM PD、15μM PD將PC3細胞阻滯在G2/M期；而將DU145和LNCaP細胞阻滯在G0/G1期。PD影響PC3細胞的細胞週期相關蛋白：E2F1，CDK2，CDK4，CDK6，CyclinD1，Cdc2，CyclinB1蛋白錶達量呈現下降趨勢，與PD對細胞週期的阻滯作用相符閤。結論PD能夠殺傷前列腺癌細胞，阻滯細胞週期進展，從而抑製腫瘤的髮展。
목적：연구래자길경적조감류단체화합물-길경조감D（platycodin D，PD）대전렬선암세포적살상효응급가능적궤제。방법배양전렬선암세포주：PC3、DU145、LNCaP，사갑기우서담（MTT）법검측PD대종류세포적존활솔적영향；류식세포의검측PD조체세포주기적능력；단백면역인적실험검측PD대주기상관단백표체적영향。결과PD살상전렬선암세포（PC3, DU145，LNCaP）적IC50치균소우30μM（PC3：11.17μM，DU145：26.13μM，LNCaP：24.6μM），수처리농도증가，살상효응경위명현，정명현제량효응관계，기중이PC3세포최위민감。PD명현조체전렬선암세포주기，10μM PD、15μM PD장PC3세포조체재G2/M기；이장DU145화LNCaP세포조체재G0/G1기。PD영향PC3세포적세포주기상관단백：E2F1，CDK2，CDK4，CDK6，CyclinD1，Cdc2，CyclinB1단백표체량정현하강추세，여PD대세포주기적조체작용상부합。결론PD능구살상전렬선암세포，조체세포주기진전，종이억제종류적발전。
Objective To evaluate the anti-tumor activity and possible mechanism(s) of Platycodin D (PD), a major saponin derived from Platycodin grandilforum, in human prostate cancer cell lines. Methods Human prostate cancer cell lines (PC3, DU145 and LNCaP cells), and the non-malignant human prostate epithelial cell line, RWPE-1, were exposed to various concentrations of PD to evaluate its cytotoxicity in vitro (via the MTT assay). Cell cycle analysis was indicated by PI staining with lfow cytometry. Cell cycle-related proteins levels were assessed by Western blotting. Results PD exerted cytotoxicity against three prostate cancer cell lines, PC3 cells, DU145 cells, and LNCaP cells, with half-maximal inhibitory concentrations (IC50) in the range of 11.17 to 26.13μmol/L. The PC3 cells were the most sensitive to PD. After being treated with PD for 48 hours, the PC3 cells were arrested in the G2/M phase, and the DU145 and LNCaP cells were arrested in the G0/G1 phase. Western blotting analysis indicated that PD exposure decreased the levels of cell cycle-related proteins, including E2F1, CDK2, CDK4, CDK6, CyclinD1, Cdc2, CyclinB1. Conclusions PD exhibits signiifcant inhibitory activities against prostate cancer cells, which provides a basis for future development of human prostate cancer chemotherapy.
