CAJ | 학술논문

目的：探讨金属硫蛋白2A（ MT2A）基因沉默对乳腺癌细胞呼吸链中细胞色素 C 氧化酶6B2亚基（ Cpy6B2）表达的影响。方法乳腺癌野生细胞株MCF-7（以下简称MCF-7）分为空白对照组和MT2A干扰组，每组各12复孔。空白对照组不转染任何遗传物质， MT2A干扰组转染shRNA-MT2A载体，采用半定量PCR法检测两组MT2A mRNA的表达水平，MT2A干扰组成功获得 MT2A基因沉默，采用实时定量PCR法分析两组Cpy6B2的表达水平。结果 MT2A干扰组中Cpy6B2的表达水平升高，与空白对照组相比，P＜0．05。结论 MT2A基因可抑制人乳腺癌细胞Cpy6B2的表达。
목적：탐토금속류단백2A（ MT2A）기인침묵대유선암세포호흡련중세포색소 C 양화매6B2아기（ Cpy6B2）표체적영향。방법유선암야생세포주MCF-7（이하간칭MCF-7）분위공백대조조화MT2A간우조，매조각12복공。공백대조조불전염임하유전물질， MT2A간우조전염shRNA-MT2A재체，채용반정량PCR법검측량조MT2A mRNA적표체수평，MT2A간우조성공획득 MT2A기인침묵，채용실시정량PCR법분석량조Cpy6B2적표체수평。결과 MT2A간우조중Cpy6B2적표체수평승고，여공백대조조상비，P＜0．05。결론 MT2A기인가억제인유선암세포Cpy6B2적표체。
Objective To investigate the effect of metallothionein 2A ( MT2A) silencing on cytochrome C oxidase 6B2 subunit (Cpy6B2) expression of breast cancer cells .Methods The breast cancer wild cell line MCF-7 (hereinafter referred as the MCF-7) was divided into the blank control group and MT 2A interference group, and each group had 12 holes.The blank control group did not transfect any genetic materials , and the MT2A interference group transfected the shRNA-MT2A vector.Using semi-quantitative PCR to detect the MT2A mRNA expression levels of the two groups .The MT2A interference group successfully obtained the MT 2A gene silencing .The real-time quantitative PCR was used to ana-lyze the expression of Cpy6B2 in the two groups.Results The results showed that the expression level of MCF-7 Cpy6B2 was increased under the MT2A gene silencing.Conclusion MT2A can inhibit the Cpy6B2 expression of breast cancer cells.