临床神经外科杂志
臨床神經外科雜誌
림상신경외과잡지
JOURNAL OF CLINICAL NEUROSURGERY
2015年
2期
118-122
,共5页
潘俊辰%徐拓野%余震南%罗正祥%赵鹏来%马骏%邹元杰%章文斌%黄庆玖%陈永严%马长艳%张岩松
潘俊辰%徐拓野%餘震南%囉正祥%趙鵬來%馬駿%鄒元傑%章文斌%黃慶玖%陳永嚴%馬長豔%張巖鬆
반준신%서탁야%여진남%라정상%조붕래%마준%추원걸%장문빈%황경구%진영엄%마장염%장암송
长链非编码RNA%胶质瘤%U87%p53
長鏈非編碼RNA%膠質瘤%U87%p53
장련비편마RNA%효질류%U87%p53
long non-coding RNA%glioma%U87%p53
目的：探索上调长链非编码RNAloc285194对胶质瘤细胞系U87细胞生长的影响。方法荧光定量PCR验证lncRNA loc285194在胶质瘤样本以及U87细胞中的表达；构建过表达lncRNA loc285194的U87稳定细胞株；CCK8检测细胞增殖能力；流式细胞仪检测凋亡能力。结果（1） lncRNA loc285194在胶质瘤样本中低表达且于肿瘤的恶性程度存在相关性；（2）上调lncRNA loc285194能抑制U87细胞的增殖；（3）上调lnc loc285194能促进细胞凋亡。结论在胶质瘤细胞中上调lncRNA loc285194能减慢胶质瘤细胞的生长，提示lncRNA loc285194可能成为一个新的治疗靶点。
目的：探索上調長鏈非編碼RNAloc285194對膠質瘤細胞繫U87細胞生長的影響。方法熒光定量PCR驗證lncRNA loc285194在膠質瘤樣本以及U87細胞中的錶達；構建過錶達lncRNA loc285194的U87穩定細胞株；CCK8檢測細胞增殖能力；流式細胞儀檢測凋亡能力。結果（1） lncRNA loc285194在膠質瘤樣本中低錶達且于腫瘤的噁性程度存在相關性；（2）上調lncRNA loc285194能抑製U87細胞的增殖；（3）上調lnc loc285194能促進細胞凋亡。結論在膠質瘤細胞中上調lncRNA loc285194能減慢膠質瘤細胞的生長，提示lncRNA loc285194可能成為一箇新的治療靶點。
목적：탐색상조장련비편마RNAloc285194대효질류세포계U87세포생장적영향。방법형광정량PCR험증lncRNA loc285194재효질류양본이급U87세포중적표체；구건과표체lncRNA loc285194적U87은정세포주；CCK8검측세포증식능력；류식세포의검측조망능력。결과（1） lncRNA loc285194재효질류양본중저표체차우종류적악성정도존재상관성；（2）상조lncRNA loc285194능억제U87세포적증식；（3）상조lnc loc285194능촉진세포조망。결론재효질류세포중상조lncRNA loc285194능감만효질류세포적생장，제시lncRNA loc285194가능성위일개신적치료파점。
Objective To investigate the long non coding RNA ( lncRNA) loc285194 expression levels in glioma and the role of lncRNA loc 285194 in human glioma u87 cell biological functions . Methods Quantitative real-time polymerase chain reactions for lncRNA-loc285194 expression were performed in 4 epilepsy brain tissues and 38 pairs of glioma tissues and adjacent normal tissues in our department .Over-expressed of lncRNA loc 285194 cell lines were constructed .Cell growth , and apoptosis were respectively tested by CCK 8 assay and flow cytometry .Western blot was also used to detected p53 protein.Result lncRNA loc285194 was significantly low-expressed in glioma tissues . Over-expressed lncRNA loc285194 inhabited human glioma U87 cells growth ( P <0.05 ) and promoted U87 cells apoptosis ( P <0 .05 ) .Conclusions lncRNA loc285194 is low-expressed in human glioma tissues . Over-expression lncRNA loc 285194 suppress glioma cells growth and promoted glioma cell apoptosis through inducing p 53 protein.It might be a new gene therapy target .
