临床荟萃
臨床薈萃
림상회췌
CLINICAL FOCUS
2015年
4期
416-420
,共5页
李静%王亮%杨红申%李亚妹%侯宏伟%李香兰%张聪瑶%李玉静
李靜%王亮%楊紅申%李亞妹%侯宏偉%李香蘭%張聰瑤%李玉靜
리정%왕량%양홍신%리아매%후굉위%리향란%장총요%리옥정
哮喘%气道重塑%多西环素%基质金属蛋白酶 9%白细胞介素 5%白细胞介素 13%肿瘤坏死因子α%肌动蛋白类
哮喘%氣道重塑%多西環素%基質金屬蛋白酶 9%白細胞介素 5%白細胞介素 13%腫瘤壞死因子α%肌動蛋白類
효천%기도중소%다서배소%기질금속단백매 9%백세포개소 5%백세포개소 13%종류배사인자α%기동단백류
asthma%airway remodeling%air remodeling%doxycycline%matrix metalloproteinase 9%interleukin-5%interleukin-13%tumor necrosis factor-alpha%actins
目的：探讨多西环素对哮喘大鼠气道炎症及重塑的预防作用及可能机制。方法将实验 SD 大鼠分为正常对照组、哮喘组、多西环素干预组。计数大鼠肺泡灌洗液中的细胞数并进行分类；检测血清中的白细胞介素5(IL-5)、白细胞介素13(IL-13)、肿瘤坏死因子α(TNF-α)的水平；检测肺组织中基质金属蛋白酶9(MMP-9)、肌动蛋白α(α-SMA)的表达；测定支气管基底膜周径(Pbm)、总管壁面积(WAt)、平滑肌面积(WAm)等反映气道壁厚度的指标，分析多西环素的影响。结果哮喘组与多西环素干预组的肺泡灌洗液细胞总数、嗜酸性粒细胞计数、支气管壁厚度、平滑肌厚度、血清 IL-5、IL-13及 TNF-α水平、肺组织 MMP-9、α-SMA 平均光度值均明显高于正常对照组，其中多西环素干预组均明显低于哮喘组(P <0.05)。结论多西环素可以影响炎症介质的生成及通过抑制 MMP-9的活性从而减轻哮喘的气道炎症及气道重塑。
目的：探討多西環素對哮喘大鼠氣道炎癥及重塑的預防作用及可能機製。方法將實驗 SD 大鼠分為正常對照組、哮喘組、多西環素榦預組。計數大鼠肺泡灌洗液中的細胞數併進行分類；檢測血清中的白細胞介素5(IL-5)、白細胞介素13(IL-13)、腫瘤壞死因子α(TNF-α)的水平；檢測肺組織中基質金屬蛋白酶9(MMP-9)、肌動蛋白α(α-SMA)的錶達；測定支氣管基底膜週徑(Pbm)、總管壁麵積(WAt)、平滑肌麵積(WAm)等反映氣道壁厚度的指標，分析多西環素的影響。結果哮喘組與多西環素榦預組的肺泡灌洗液細胞總數、嗜痠性粒細胞計數、支氣管壁厚度、平滑肌厚度、血清 IL-5、IL-13及 TNF-α水平、肺組織 MMP-9、α-SMA 平均光度值均明顯高于正常對照組，其中多西環素榦預組均明顯低于哮喘組(P <0.05)。結論多西環素可以影響炎癥介質的生成及通過抑製 MMP-9的活性從而減輕哮喘的氣道炎癥及氣道重塑。
목적：탐토다서배소대효천대서기도염증급중소적예방작용급가능궤제。방법장실험 SD 대서분위정상대조조、효천조、다서배소간예조。계수대서폐포관세액중적세포수병진행분류；검측혈청중적백세포개소5(IL-5)、백세포개소13(IL-13)、종류배사인자α(TNF-α)적수평；검측폐조직중기질금속단백매9(MMP-9)、기동단백α(α-SMA)적표체；측정지기관기저막주경(Pbm)、총관벽면적(WAt)、평활기면적(WAm)등반영기도벽후도적지표，분석다서배소적영향。결과효천조여다서배소간예조적폐포관세액세포총수、기산성립세포계수、지기관벽후도、평활기후도、혈청 IL-5、IL-13급 TNF-α수평、폐조직 MMP-9、α-SMA 평균광도치균명현고우정상대조조，기중다서배소간예조균명현저우효천조(P <0.05)。결론다서배소가이영향염증개질적생성급통과억제 MMP-9적활성종이감경효천적기도염증급기도중소。
ABSTRACT:Objective To investigate the effect of doxycycline on airway inflammation and remodeling in asthmatic rats.Methods Thirty-three male Sprague Dawley rats were randomly divided into three groups:control group,asthma group and doxycycline intervention group.Total cell number and different cell number in BALF were counted.The concentrations of serum IL-5,IL-13 were assayed by ELISA,and the concentration of serum TNF-αwas determined by radioimmunoassay.The expression of MMP-9 and α-SMA in lung tissues were observed by immunohistochemistry.Lung tissues were sliced and stained with HE.These parameters such as bronchial basement membrane perimeter (Pbm),total bronchial wall area (WAt)and smooth muscle area (WAm),which reflected the thickness of airway wall,were measured by image analysis system.Results The count of total cells and eosinophils significantly increased in asthma group and doxycycline intervention group than in control group(P <0.05),and the count of total cells and eosinophils significantly decreased in doxycycline intervention group than in asthma group (P <0.05).The thickness of bronchial wall and smooth muscle layer were significantly thicker in asthma group and doxycycline intervention group than in normal group (P < 0.05 ),and the thickness of bronchial wall and smooth muscle layer were significantly thinner in doxycycline intervention group than in asthma group (P < 0.05 ).The concentrations of serum IL-5,IL-13 and TNF-α were significantly higher in asthma group (38.63 ± 6.64)ng/L and doxycycline intervention group (20.42±4.50)ng/L than in control group (1 5.92 ±3.66)ng/L(P <0.05),and the concentrations of serum IL-5,IL-13 and TNF-α were significantly lower in doxycycline intervention group than in asthma group (P < 0.05 ).The average optical density of MMP-9 and α-SMA of asthma group (0.52 ± 0.06 )and doxycycline intervention group (0.39±0.06)were significantly higher than those of control group (0.24±0.06)(P <0.05 ),and the average optical density of MMP-9 andα-SMA were significantly lower in doxycycline intervention group than in asthma group(P <0.05).Conclusion Doxycycline can decrease asthmatic airway inflammation by inhibiting the activity of MMP-9 and influencing the production of inflammatory mediators and improve airway remodeling of asthma through inhibiting the activity of MMP-9.
