CAJ | 학술논문

目的：：探讨电化学发光法( ECLIA)和酶联免疫吸附法( ELISA)在乙型肝炎病毒血清标志物检测中的差异。方法：分别使用ECLIA和ELISA法对2013年1-12月广州医学院荔湾医院收集的标本进行HBV-M检测,分析两种检测方法的差异及临床应用价值。结果：两种检测方法测定HBsAg的阳性率比较,差异无统计学意义(P>0.05),有较好的一致性(KaPp ＝0.96)。 ELISA法测定HBsAb的S/CO比值>11.0者156例,其中152例ECLIA法测定值>100 mlU/mL,一致性达97.4％。结论：ELISA法测定HBsAg与ECllA法测定结果一致；与ECLIA相比,ELISA测定的HBsAb具有肯定的免疫保护作用的S/CO比值>11.0；不具有免疫保护作用的S/CO比值<5。
목적：：탐토전화학발광법( ECLIA)화매련면역흡부법( ELISA)재을형간염병독혈청표지물검측중적차이。방법：분별사용ECLIA화ELISA법대2013년1-12월엄주의학원려만의원수집적표본진행HBV-M검측,분석량충검측방법적차이급림상응용개치。결과：량충검측방법측정HBsAg적양성솔비교,차이무통계학의의(P>0.05),유교호적일치성(KaPp ＝0.96)。 ELISA법측정HBsAb적S/CO비치>11.0자156례,기중152례ECLIA법측정치>100 mlU/mL,일치성체97.4％。결론：ELISA법측정HBsAg여ECllA법측정결과일치；여ECLIA상비,ELISA측정적HBsAb구유긍정적면역보호작용적S/CO비치>11.0；불구유면역보호작용적S/CO비치<5。
Objective:To investigate the differences between electrochemiluminescence immunoassay ( ECLIA) and enzyme-linked immunosorbent assay ( ELISA) in the detection of hepatitis B virus ( HBV) serum markers.Methods:The serum samples collected in our hospital from January to December in 2013 were tested by ECLIA and ELISA respectively for HBV-M detection in order to analyze the differences and clinical value of the two detection methods.Results:The HBsAg positive rate detected by ELISA and ECLIA did not differ statistically (P>0.05),and the results showed a good consistency between both approaches (KaPp=0.96).The S/CO ratios of HBsAb tested by ELISA of 156 cases were >11.0,including 152 cases with>100 mlU/mL values tested by ECLIA,and the consistency reached 97. 4%. Conclusion:The results of HBsAg detected by ELISA and ECLIA were consistent. Compared with ECLIA, the S/CO ratio of HBsAb detected by ELISA with positive immune protective effect was >11.0;and S/CO ratio without immune protective effect was<5.