CAJ | 학술논문

目的：：探讨骨桥蛋白(osteopontin,OPN)与骨涎蛋白(bonesialoprotein,BSP)在非负荷期非埋植型种植体周围骨界面改建中的作用。方法：选用健康雄性杂种犬8只,在其下颌植入非埋植型种植体,分不同时期处死动物。采用免疫组化方法,对不同时期非埋植型种植体-骨界面OPN与BSP进行动态观察,研究种植体-骨界面中OPN与BSP在界面改建过程中的表达变化。结果：种植义齿植入后在非负荷期,随着种植体周围骨组织发生改建,种植体-骨界面表达OPN和BSP有明显规律性,代表其染色强度的灰度值OPN从79.53±2.33增加到122.17±0.65；BSP从79.71±0.55增加到122.82±0.85。1周时界面OPN与BSP都有表达,2周时表达达到高峰,1月时逐渐下降,3月时趋于正常(均P<0.05)。结论：非埋植型种植体在非负荷期,种植体-骨界面OPN与BSP表达的变化有明显的规律性,且OPN与BSP能促进其界面的改建。在维持种植体与骨界面之间的骨性结合形成中起重要作用。
목적：：탐토골교단백(osteopontin,OPN)여골연단백(bonesialoprotein,BSP)재비부하기비매식형충식체주위골계면개건중적작용。방법：선용건강웅성잡충견8지,재기하합식입비매식형충식체,분불동시기처사동물。채용면역조화방법,대불동시기비매식형충식체-골계면OPN여BSP진행동태관찰,연구충식체-골계면중OPN여BSP재계면개건과정중적표체변화。결과：충식의치식입후재비부하기,수착충식체주위골조직발생개건,충식체-골계면표체OPN화BSP유명현규률성,대표기염색강도적회도치OPN종79.53±2.33증가도122.17±0.65；BSP종79.71±0.55증가도122.82±0.85。1주시계면OPN여BSP도유표체,2주시표체체도고봉,1월시축점하강,3월시추우정상(균P<0.05)。결론：비매식형충식체재비부하기,충식체-골계면OPN여BSP표체적변화유명현적규률성,차OPN여BSP능촉진기계면적개건。재유지충식체여골계면지간적골성결합형성중기중요작용。
Objective:To investigate the role of osteopontin (OPN) and bonesialoprotein (BSP) in the remodeling bone interface around non-submerged implant during unloaded period. Methods: Eight healthy male mongrel dogs were used to build the animal model. Non-submerged implants were implanted into the bilateral mandibles of the dogs.The dogs were sacrificed in different stages after the procedure.Immunohistochemistry was used to observe the dynamic expression of OPN and BSP of non-submerged implant-bone interface in different stages,so as to study the change in expression of OPN and BSP in remodeling implant-bone interface. Results:According to immunohistochemical study,implant denture remodeled along with bone tissue around the implant after being implanted during unloaded period. The expression of OPN and BSP in the implant-bone interface showed significant regularity.The gray level (representing staining intensity) of OPN was enhanced from 79.53± 2.33 to 122.17±0.65;and that of BSP,from 79.71±0.55 to 122.82±0.85(P<0.05).The expressions of OPN and BSP were observed after 1 week, peaked after 2 weeks, decreased gradually after 1 month, and dropped to the normal level after 3 months. Conclusion:The expression changes of OPN and BSP in non-submerged implant interface have significant regularity during unloaded period.Besides,OPN and BSP can stimulate the remodeling of the interface,and have an important role in maintaining the formation of bony union between implant and bone interface.