CAJ | 학술논문

目的：：探讨不同添加剂对聚乳酸-聚乙二醇酸( PLGA)包裹牛血清白蛋白( BSA)制备的BSA-PLGA微球特性的影响。方法：采用复乳-溶剂挥发法制备BSA-PLGA微球，显微测量微球粒径，以微量BCA 法测定微球的蛋白含量并计算包封率，进行体外释放，测定微球的累积释放量。探索添加剂PEG6000、Tween-20、Trehalose对微球粒径、包封率、突释量和累积释放量的影响。结果：加入添加剂制备的微球，粒径增大，突释降低，载药量、包封率、累积释放量均有所提高，且呈浓度依赖性(P<0.05)。结论：通过加入适当种类和浓度的添加剂，可以得到较高包封率和累积释放量、较小突释、适当载药量和粒径的BSA-PLGA微球。
목적：：탐토불동첨가제대취유산-취을이순산( PLGA)포과우혈청백단백( BSA)제비적BSA-PLGA미구특성적영향。방법：채용복유-용제휘발법제비BSA-PLGA미구，현미측량미구립경，이미량BCA 법측정미구적단백함량병계산포봉솔，진행체외석방，측정미구적루적석방량。탐색첨가제PEG6000、Tween-20、Trehalose대미구립경、포봉솔、돌석량화루적석방량적영향。결과：가입첨가제제비적미구，립경증대，돌석강저，재약량、포봉솔、루적석방량균유소제고，차정농도의뢰성(P<0.05)。결론：통과가입괄당충류화농도적첨가제，가이득도교고포봉솔화루적석방량、교소돌석、괄당재약량화립경적BSA-PLGA미구。
Objective:To investigate the effects of different additives on the characteristics of BSA-PLGA sustained-release microspheres prepared by polylactic acid-glycolic acid ( PLGA) encapsulation of bovine serum albumin ( BSA ) . Methods:BSA-PLGA microspheres were prepared by double emulsion/solvent evaporation method.The diameter of microspheres was measured by microscope. The protein content of microspheres was determined by micro BCA assay,and the encapsulation efficiency was calculated. Microspheres were released in vitro and the total release amount was detected.The effects of additives PEG6000,Tween-20 and Trehalose on the diameter,encapsulation efficiency,burst release amount and total release amount of microspheres were evaluated. Results:At the presence of additives, the diameter, drug loading capacity, encapsulation efficiency and total release amount of the prepared microspheres increased significantly while the burst release decreased; all these changes were dose-dependent (P<0.05).Conclusion:BSA-PLGA microspheres with relatively high encapsulation efficiency and total release amount,low burst release,proper drug loading and particle diameter could be produced by adding suitable types and concentrations of additives.