中国民族民间医药
中國民族民間醫藥
중국민족민간의약
CHINESE JOURNAL OF ETHNOMEDICINE AND ETHNOPHARMACY
2015年
7期
19-20
,共2页
秦晴%阙金花%张玉琴%李煌%徐伟%褚克丹
秦晴%闕金花%張玉琴%李煌%徐偉%褚剋丹
진청%궐금화%장옥금%리황%서위%저극단
海风藤%胶原诱导性关节炎%NF-κB%IκB%IκK
海風籐%膠原誘導性關節炎%NF-κB%IκB%IκK
해풍등%효원유도성관절염%NF-κB%IκB%IκK
Piperis kadsurae caulis%collagen-induced rheumatoid arthritis%NF-κB%IκB%IκK
目的：研究海风藤正丁醇提取物对胶原诱导性关节炎大鼠滑膜组织中NF－κB、IκB和IκK的影响，探讨其治疗作用机制。方法：通过建立胶原诱导性关节炎（CIA）大鼠模型，观察大鼠足趾肿胀度的变化，蛋白印迹法检测大鼠踝关节滑膜组织中NF－κB、IκB和IκK的表达水平。结果：海风藤正丁醇提取物能改善大鼠活动减少、精神不佳等一般状态，下调滑膜组织中 NF－κB、IκK的表达及上调滑膜组织中IκB的表达。结论：海风藤正丁醇提取物对类风湿性关节炎有一定的治疗作用，其机理与调节滑膜组织中NF－κB、IκB和IκK的表达水平有关。
目的：研究海風籐正丁醇提取物對膠原誘導性關節炎大鼠滑膜組織中NF－κB、IκB和IκK的影響，探討其治療作用機製。方法：通過建立膠原誘導性關節炎（CIA）大鼠模型，觀察大鼠足趾腫脹度的變化，蛋白印跡法檢測大鼠踝關節滑膜組織中NF－κB、IκB和IκK的錶達水平。結果：海風籐正丁醇提取物能改善大鼠活動減少、精神不佳等一般狀態，下調滑膜組織中 NF－κB、IκK的錶達及上調滑膜組織中IκB的錶達。結論：海風籐正丁醇提取物對類風濕性關節炎有一定的治療作用，其機理與調節滑膜組織中NF－κB、IκB和IκK的錶達水平有關。
목적：연구해풍등정정순제취물대효원유도성관절염대서활막조직중NF－κB、IκB화IκK적영향，탐토기치료작용궤제。방법：통과건립효원유도성관절염（CIA）대서모형，관찰대서족지종창도적변화，단백인적법검측대서과관절활막조직중NF－κB、IκB화IκK적표체수평。결과：해풍등정정순제취물능개선대서활동감소、정신불가등일반상태，하조활막조직중 NF－κB、IκK적표체급상조활막조직중IκB적표체。결론：해풍등정정순제취물대류풍습성관절염유일정적치료작용，기궤리여조절활막조직중NF－κB、IκB화IκK적표체수평유관。
Objective To investigate the therapeutic effect of Piperis kadsurae caulis extraction on collagen-induced rheumatoid arthritis rats,and its effects on NF-κB,IκB and IκK in synovial tissue,and to explore its therapeutic mechanism.Methods CIA rat model was induced by injecting bovine type II collagen.The swelling level of paw was observed and the expression of NF-κB,IKB and IκK were evaluated by Western blotting.Results The ethyl acetate extraces of Piperis kadsurae caulis could inhibit the paw swell-ing and down-regulate the expression of NF-κB,IκB and IκK.Conclusion The ethyl acetate extraces of Piperis kadsurae caulis have certain therapeutic on rheumatoid arthritis by reducing the expression of NF-κB,IκB and IκK in synovial tissue.
