CAJ | 학술논문

白菜型冬春性油菜杂交后代抗寒性变异分析
백채형동춘성유채잡교후대항한성변이분석
Analyses of cold tolerance variations in filial generations of winter and spring turnip rape (Brassica rapa L .)

万方数据

干旱地区农业研究榦旱地區農業研究 간한지구농업연구
AGRICULTURAL RESEARCH IN THE ARID AREAS
2015年 2期 95-101 ,共7页

杨刚%王丽萍%孙万仓%曾秀存%刘自刚%李学才%方彦%武军艳%赵艳宁%董红业%王月

양강%왕려평%손만창%증수존%류자강%리학재%방언%무군염%조염저%동홍업%왕월

白菜型油菜%杂交后代%抗寒性%生理生化特性%越冬率白菜型油菜%雜交後代%抗寒性%生理生化特性%越鼕率
백채형유채%잡교후대%항한성%생리생화특성%월동솔
Brassica rapa L .%Filial generations%Cold tolerance%Physiological indexes%Overwintering rates

选用抗寒性强的白菜型冬油菜（陇油7号和陇油9号）与优质春油菜（Parkland）杂交的后代群体（F1，F2和 BC1）为研究对象，以降温前后的油菜 SOD 、POD 、CAT 、可溶性蛋白和 MDA 等5个生理生化特性的变异情况为依据，结合越冬率数据对杂交后代进行抗寒性分析。研究表明：降温后，所有单株的生理生化指标都增加。降温前SOD 、POD 、CAT 、可溶性蛋白和 MDA 值分别介于131．16～489．50 U?g －1、8．59～68．44 U?g －1、2．63～20．85 U?g －1、2．53～7．80 mg?g －1和3．69～13．67 mg?g －1 。降温后，分别为290．87～796．88 U?g －1、22．63～101．5 U?g －1、8．89～48．30 U?g －1、4．46～10．68 mg?g －1和5．77～21．25 mg?g －1 。生理生化指标和越冬率均发生分离，F1代生理生化变异系数小，而以冬性亲本为母本的后代越冬率（70．27％）高于春性亲本为母本的后代（64．48％）。 F2代和 BC1代变异较大，其中 BC1代中以冬性亲本为轮回亲本的群体越冬率（71．43％～76．19％）明显高于以春性亲本为轮回亲本群体的越冬率（38．46％～43．75％）。相关性分析表明这5个生理生化特性与越冬率极显著相关，相关系数分别为0．597、0．757、0．665、0．567和－0．663。分析结果表明各项生理生化指标结合越冬率对冬春杂交后代的抗寒性分析是可行的，能够筛选出抗寒优良单株，为白菜型冬油菜品质改良提供基础材料。
선용항한성강적백채형동유채（롱유7호화롱유9호）여우질춘유채（Parkland）잡교적후대군체（F1，F2화 BC1）위연구대상，이강온전후적유채 SOD 、POD 、CAT 、가용성단백화 MDA 등5개생리생화특성적변이정황위의거，결합월동솔수거대잡교후대진행항한성분석。연구표명：강온후，소유단주적생리생화지표도증가。강온전SOD 、POD 、CAT 、가용성단백화 MDA 치분별개우131．16～489．50 U?g －1、8．59～68．44 U?g －1、2．63～20．85 U?g －1、2．53～7．80 mg?g －1화3．69～13．67 mg?g －1 。 강온후，분별위290．87～796．88 U?g －1、22．63～101．5 U?g －1、8．89～48．30 U?g －1、4．46～10．68 mg?g －1화5．77～21．25 mg?g －1 。 생리생화지표화월동솔균발생분리，F1대생리생화변이계수소，이이동성친본위모본적후대월동솔（70．27％）고우춘성친본위모본적후대（64．48％）。 F2대화 BC1대변이교대，기중 BC1대중이동성친본위륜회친본적군체월동솔（71．43％～76．19％）명현고우이춘성친본위륜회친본군체적월동솔（38．46％～43．75％）。 상관성분석표명저5개생리생화특성여월동솔겁현저상관，상관계수분별위0．597、0．757、0．665、0．567화－0．663。분석결과표명각항생리생화지표결합월동솔대동춘잡교후대적항한성분석시가행적，능구사선출항한우량단주，위백채형동유채품질개량제공기출재료。
The winter and spring turnip rape parents (Longyou 7 ,Longyou 9 and Parkland) and their filial genera-tions ,significantly differing in cold tolerance and quality ,were comprehensively analyzed .Cold tolerance variations were examined on the basis of five physiological indexes and overwintering rates before and after cooling .The results showed that the physiological indexes of all individuals were increased after cooling .The SOD ,POD ,CAT ,soluble protein and MDA values were 131 .16 ~ 489 .50 U?g - 1 ,8 .59 ~ 68 .44 U?g - 1 ,2 .63 ~ 20 .85 U?g - 1 ,2 .53 ~ 7 .80 mg?g - 1 and 3 .69 ~ 13 .67 mg?g - 1 before cooling ,but reached to 290 .87 ~ 796 .88 U?g - 1 ,22 .63 ~ 101 .5 U?g - 1 ,8 .89 ~ 48 .30 U?g - 1 ,4 .46 ~ 10 .68 mg?g - 1 and 5 .77 ~ 21 .25 mg?g - 1 respectively after the treatment .In addition ,physiological in-dexes and overwintering rates were segregated .The variation of physiological coefficient in F1 generation was small ,and the overwintering rates of filial generations of winter turnip rape for female parents (70 .27% ) were higher than those of the spring parent for the female parent (64 .48% ) .The variations in F2 and BC1 generations were large ,and overwinter-ing rates of a group of winter parents for the recurrent parent (71 .43% ~ 76 .19% ) were significantly higher than the groups of spring parents as the recurrent parent (38 .46% ~ 43 .75% ) in BC1 .It was further found that significant corre-lations between the five physiological characteristics and overwintering rates could be identified .Correlation coefficients were 0 .597 ,0 .757 ,0 .665 ,0 .567 and -0 .663 respectively .In conclusion ,it was feasible to analyze cold tolerance characteristics using these physiological indexes ,which could reveal the differences of cold tolerance indexes and over-wintering rates in different filial generations ,and could provide good base materials for quality improvement of winter turnip rape .