中国神经精神疾病杂志
中國神經精神疾病雜誌
중국신경정신질병잡지
CHINESE JOURNAL OF NERVOUS AND MENTAL DISEASES
2015年
2期
92-97
,共6页
郑璐%范玉华%蓝琳芳%姬晓昙
鄭璐%範玉華%藍琳芳%姬曉曇
정로%범옥화%람림방%희효담
高血压%脑白质病%认知障碍
高血壓%腦白質病%認知障礙
고혈압%뇌백질병%인지장애
Hypertension%White matter lesion%Cognition dysfunction
目的：探讨瑞舒伐他汀钙对慢性高血压大鼠的脑白质病变及其空间认知功能障碍的影响。方法雄性Sprague-DawIey大鼠49只，随机分为假手术组、高血压组和瑞舒伐他汀钙治疗组（10 mg/kg）。高血压组和瑞舒伐他汀钙治疗组采用双肾双夹法制作易卒中型肾血管性高血压大鼠（stroke-prone renovascular hyperten?sive rat, RHRSP）模型，定期监测血压。水迷宫实验检测空间记忆功能；Luxol fast blue染色观察脑白质疏松程度；免疫荧光法观察额叶皮层β-淀粉样蛋白（β-amyloid，Aβ）在大脑皮层的沉积分布；原位末端转移酶标记染色法（TdT-mediated dUTP-biotin nick end labeling, TUNEL）观察额叶皮层细胞凋亡水平。结果RHRSP大鼠造模后血压逐渐升高，明显高于假手术组(P<0.01)；瑞舒伐他汀钙较高血压组逃避潜伏期时间明显缩短(P<0.01)，3组大鼠跨越平台次数分别为(4.55±1.23)、(1.00±0.80)、(3.79±0.95)，差异有统计学意义(P<0.01)。与高血压组相比，瑞舒伐他汀钙可改善脑白质疏松程度(P<0.01)，减少额叶皮层区Aβ沉积（17.47±3.59 vs.4.42±1.57，P<0.01）及细胞凋亡（37.84±4.73 vs.14.42±2.43，P<0.01）。结论瑞舒伐他汀钙可改善慢性高血压大鼠的脑白质病变，减少额叶皮层区Aβ沉积以及细胞凋亡从而保护大鼠的空间记忆功能。
目的：探討瑞舒伐他汀鈣對慢性高血壓大鼠的腦白質病變及其空間認知功能障礙的影響。方法雄性Sprague-DawIey大鼠49隻，隨機分為假手術組、高血壓組和瑞舒伐他汀鈣治療組（10 mg/kg）。高血壓組和瑞舒伐他汀鈣治療組採用雙腎雙夾法製作易卒中型腎血管性高血壓大鼠（stroke-prone renovascular hyperten?sive rat, RHRSP）模型，定期鑑測血壓。水迷宮實驗檢測空間記憶功能；Luxol fast blue染色觀察腦白質疏鬆程度；免疫熒光法觀察額葉皮層β-澱粉樣蛋白（β-amyloid，Aβ）在大腦皮層的沉積分佈；原位末耑轉移酶標記染色法（TdT-mediated dUTP-biotin nick end labeling, TUNEL）觀察額葉皮層細胞凋亡水平。結果RHRSP大鼠造模後血壓逐漸升高，明顯高于假手術組(P<0.01)；瑞舒伐他汀鈣較高血壓組逃避潛伏期時間明顯縮短(P<0.01)，3組大鼠跨越平檯次數分彆為(4.55±1.23)、(1.00±0.80)、(3.79±0.95)，差異有統計學意義(P<0.01)。與高血壓組相比，瑞舒伐他汀鈣可改善腦白質疏鬆程度(P<0.01)，減少額葉皮層區Aβ沉積（17.47±3.59 vs.4.42±1.57，P<0.01）及細胞凋亡（37.84±4.73 vs.14.42±2.43，P<0.01）。結論瑞舒伐他汀鈣可改善慢性高血壓大鼠的腦白質病變，減少額葉皮層區Aβ沉積以及細胞凋亡從而保護大鼠的空間記憶功能。
목적：탐토서서벌타정개대만성고혈압대서적뇌백질병변급기공간인지공능장애적영향。방법웅성Sprague-DawIey대서49지，수궤분위가수술조、고혈압조화서서벌타정개치료조（10 mg/kg）。고혈압조화서서벌타정개치료조채용쌍신쌍협법제작역졸중형신혈관성고혈압대서（stroke-prone renovascular hyperten?sive rat, RHRSP）모형，정기감측혈압。수미궁실험검측공간기억공능；Luxol fast blue염색관찰뇌백질소송정도；면역형광법관찰액협피층β-정분양단백（β-amyloid，Aβ）재대뇌피층적침적분포；원위말단전이매표기염색법（TdT-mediated dUTP-biotin nick end labeling, TUNEL）관찰액협피층세포조망수평。결과RHRSP대서조모후혈압축점승고，명현고우가수술조(P<0.01)；서서벌타정개교고혈압조도피잠복기시간명현축단(P<0.01)，3조대서과월평태차수분별위(4.55±1.23)、(1.00±0.80)、(3.79±0.95)，차이유통계학의의(P<0.01)。여고혈압조상비，서서벌타정개가개선뇌백질소송정도(P<0.01)，감소액협피층구Aβ침적（17.47±3.59 vs.4.42±1.57，P<0.01）급세포조망（37.84±4.73 vs.14.42±2.43，P<0.01）。결론서서벌타정개가개선만성고혈압대서적뇌백질병변，감소액협피층구Aβ침적이급세포조망종이보호대서적공간기억공능。
Objective To investigate the potential effects of rosuvastatin on white matter lesion and spatial memo?ry function in chronic hypertensive rats. Methods Fourty-nine male Sprague-Dawley rats were randomly divided into sham-operation group, vehicle-treated group, and rosuvastatin-treated group (10 mg/kg).A model of stroke-prone reno?vascular hypertensive rat (RHRSP) was induced by using the two-kidney two clip method in the vehicle-treated group and the rosuvastatin-treated group. Blood pressure was monitored regularly. Morris water maze experiment was conduct?ed to assess spatial memory function. Luxol fast blue stanning was used to examine the degree of leukoaraiosis. Immuno?fluorescence and electron microscopy was used to detectβ-amyloid deposits. TUNEL staining was used to assess apopto?sis. Results The blood pressure of RHRSP increased progressively after operation.Blood pressure was significantly high?er in RHRSP than in sham-operation group (P<0.01). The escape latencies of the rosuvastatin-treated group were mark?edly shorter in RHRSP than in sham-operation group (P<0.01). The numbers of crossing hidden platform in the 3 groups of rats were 4.55±1.23, 1.00±0.80 and 3.79±0.95 times. There were significantly differences in numbers of crossing hid? den platform among three groups (P<0.01). Luxol fast blue stanning showed that the grading scores for WML were lower in the rosuvastatin-treated than in the vehicle-treated group (P<0.01). Rosuvastatin significantly decreased the burden of Aβdeposits(17.47±3.59 vs. 4.42±1.57,P<0.01)and the TUNEL+cells(37.84±4.73 vs. 14.42±2.43,P<0.01)in the fron?tal cortex when compared with the vehicle-treated group. Conclusions Rosuvastatin may ameliorate spatial memory func?tion through attenuation of white matter lesion, Aβdeposits and apoptosis .
