福建畜牧兽医
福建畜牧獸醫
복건축목수의
FUJIAN JOURNAL OF ANIMAL HUSBANDRY AND VETERINARY
2015年
2期
1-3,4
,共4页
钼%小鼠%雌激素
鉬%小鼠%雌激素
목%소서%자격소
molybdenum%mice%estrogen
本试验主要探讨金属元素钼(Mo)对F1代雌性小鼠雌激素的影响。试验选择健康昆明系ICR F1雌性小白鼠20只,体重30~40 g,适应5 d后,随机分为4组。基础日粮相同,试验组分别在其饮水中加入100、200和400 mg/L钼酸钠,对照组给予正常饮水。喂养7周后,测量其体重并记录。采用眼球摘除采血法采集血液制备血清,测定小鼠的肝脏、肾脏、脾脏,计算肝脏、肾脏、脾脏的脏器指数。采用发光免疫技术测定小鼠血清雌二醇水平。
本試驗主要探討金屬元素鉬(Mo)對F1代雌性小鼠雌激素的影響。試驗選擇健康昆明繫ICR F1雌性小白鼠20隻,體重30~40 g,適應5 d後,隨機分為4組。基礎日糧相同,試驗組分彆在其飲水中加入100、200和400 mg/L鉬痠鈉,對照組給予正常飲水。餵養7週後,測量其體重併記錄。採用眼毬摘除採血法採集血液製備血清,測定小鼠的肝髒、腎髒、脾髒,計算肝髒、腎髒、脾髒的髒器指數。採用髮光免疫技術測定小鼠血清雌二醇水平。
본시험주요탐토금속원소목(Mo)대F1대자성소서자격소적영향。시험선택건강곤명계ICR F1자성소백서20지,체중30~40 g,괄응5 d후,수궤분위4조。기출일량상동,시험조분별재기음수중가입100、200화400 mg/L목산납,대조조급여정상음수。위양7주후,측량기체중병기록。채용안구적제채혈법채집혈액제비혈청,측정소서적간장、신장、비장,계산간장、신장、비장적장기지수。채용발광면역기술측정소서혈청자이순수평。
To investigate the effects of metal molybdenum on estrogen of the F1 generation of female mice,20 healthy ICR rats,30~40 g,were randomly allotted to four groups.Sodium molybdate was administrated to mice at different doses (aqueous solution with 0 mg/L Mo,100 mg/L Mo,200 mg/L Mo,400 mg/L Mo)for seven weeks,the control group received normal drinking water.During the test animals, the situation of all the animals was observed and recorded.After 7 weeks of feeding,body weight was measured and recorded.Prepara﹣tion of serum was under blood collection by enucleation,and the liver,kidney and spleen of mice were determined.And the organ index of liver,kidney and spleen were calculated.The levels of estradiol were determined by immunoassay technology.