CAJ | 학술논문

目的：观察宫颈上皮内瘤样病变与人乳头瘤病毒感染的关系。方法选取妇科门诊就诊患者中经阴道镜下宫颈活检病理检查确诊为CIN的患者共263例，均对其检测HPV分型。HPV分型的检测采用PCR扩增、基因芯片探针杂交分型检测法。结果 HPV感染率随着CIN级别进展逐渐升高。CINⅠ级主要为LR-HPV感染；CINⅡ-Ⅲ主要为HR-HPV感染，尤其是HPV-16、18。HPV-16、18感染率在CINⅠ与CINⅡ、CINⅡ与CINⅢ之间差异均具有统计学意义(P<0.01)。结论 HPV感染率随着CIN级别的增高而上升,HPV感染的基因型决定了CIN病变的级别,LR-HPV主要引起轻度鳞状上皮内病变，相当于CINⅠ级，HPV基因型主要为HPV-11、6。HR-HPV主要引起高度鳞状上皮内病变，相当于CINⅡ-Ⅲ，HPV基因型主要为HPV-16、18、58。因此，对有性生活的女性开展HPV分型检测，结合宫颈液基薄层细胞制片法(TCT)检查，发现阳性及时行阴道镜下宫颈活检，及时有效地发现宫颈上皮内瘤样病变(CIN)并进行干预治疗，能降低宫颈癌的发病率和死亡率。
목적：관찰궁경상피내류양병변여인유두류병독감염적관계。방법선취부과문진취진환자중경음도경하궁경활검병리검사학진위CIN적환자공263례，균대기검측HPV분형。HPV분형적검측채용PCR확증、기인심편탐침잡교분형검측법。결과 HPV감염솔수착CIN급별진전축점승고。CINⅠ급주요위LR-HPV감염；CINⅡ-Ⅲ주요위HR-HPV감염，우기시HPV-16、18。HPV-16、18감염솔재CINⅠ여CINⅡ、CINⅡ여CINⅢ지간차이균구유통계학의의(P<0.01)。결론 HPV감염솔수착CIN급별적증고이상승,HPV감염적기인형결정료CIN병변적급별,LR-HPV주요인기경도린상상피내병변，상당우CINⅠ급，HPV기인형주요위HPV-11、6。HR-HPV주요인기고도린상상피내병변，상당우CINⅡ-Ⅲ，HPV기인형주요위HPV-16、18、58。인차，대유성생활적녀성개전HPV분형검측，결합궁경액기박층세포제편법(TCT)검사，발현양성급시행음도경하궁경활검，급시유효지발현궁경상피내류양병변(CIN)병진행간예치료，능강저궁경암적발병솔화사망솔。
Objective To observe the relationship of papilloma virus infection of human neoplasia and cervical intraepithelial.Methods To select gynecological outpatients by colposcopy cervical biopsy pathology examination conifrmed the diagnosis of 263 patients with CIN were divided into type, on the detection of HPV. Detection of HPV genotyping by PCR ampliifcation, gene chip probe hybridization genotyping method.Results The infection rate of HPV CIN level increased gradually with the progress of. CIN grade I mainly for LR-HPV infection; CIN II - III mainly for HR-HPV infection, especially HPV-16,18. Between CIN I and CIN II, CIN II and CIN III were highly statistically signiifcant difference in the infection rate of HPV-16,18 (P<0.01).Conclusion HPV infection rate increased with the increase of CIN level, genotype HPV infection determines the CIN lesion level, LR-HPV is mainly caused by mild squamous intraepithelial lesion, equivalent to CIN grade, HPV gene type is mainly HPV-11, 6. HR-HPV is mainly caused by the high grade squamous intraepithelial lesion, the equivalent of CIN II - III, HPV gene type is mainly HPV-16, 18, 58. Therefore, the sexual life of women to carry out the HPV genotyping, combined with the production of cervical liquid based thin-layer cell method (TCT) inspection, timely found positive colposcopic biopsy, timely and effective detection of cervical intraepithelial neoplasia (CIN) and intervention treatment, can reduce cervical cancer incidence and mortality.