CAJ | 학술논문

酶抑制法是最具前景的农药残留快速检测方法，但存在灵敏度和准确性方面的缺陷。新酶源的发掘和研究是酶抑制法发展的关键。通过同源建模及分子对接技术，对不同物种的乙酰胆碱酯酶与不同农药分子的敏感性进行了虚拟筛选，并分析了可能影响农药敏感性的关键氨基酸残基，为农药残留检测用酶的选择以及采用定点突变技术对酶进行改造的可行性提供科学依据。
매억제법시최구전경적농약잔류쾌속검측방법，단존재령민도화준학성방면적결함。신매원적발굴화연구시매억제법발전적관건。통과동원건모급분자대접기술，대불동물충적을선담감지매여불동농약분자적민감성진행료허의사선，병분석료가능영향농약민감성적관건안기산잔기，위농약잔류검측용매적선택이급채용정점돌변기술대매진행개조적가행성제공과학의거。
Enzyme inhibition method is the most promising of rapid determination of pesticide residues, but with defective sensitivity and accuracy. The discovery and research of new enzymes are the key to the development of enzyme inhibition method. The paper finished the virtual screening of acetylcholinesterase sensitive to pesticides from different species, and analyzed the critical amino acid residues for the sensitivity to pesticides based on a new strategy of homology modeling and molecular docking technology. The result would provide scientific basis for choosing the enzymes in detection of pesticide residues and for carrying out molecule rational design of novel target enzymes using site-specific mutagenesis.