CAJ | 학술논문

水稻是重要的粮食作物,其产量高低与病虫害等因素密切相关,由黄单胞杆菌引起的水稻白叶枯病是导致水稻产量减少的主要病害之一。控制白叶枯病最安全、经济有效的方法是培育抗病品种,隐性抗病基因xa5是重要的水稻白叶枯病抗性基因。通过构建显性及隐性 xa5基因的原核表达载体(PGEX-4T-1-Xa5和PGEX-4T-1-xa5),转化大肠杆菌BL21,经0.2 mmol/L IPTG 28℃诱导4 h,表达显性Xa5和隐性xa5蛋白；结合western-blotting分析,结果表明表达的蛋白是带有GST标签的融合蛋白,最后利用谷胱甘肽亲和层析柱将蛋白纯化,为后期研究xa5基因的功能提供基础。
수도시중요적양식작물,기산량고저여병충해등인소밀절상관,유황단포간균인기적수도백협고병시도치수도산량감소적주요병해지일。공제백협고병최안전、경제유효적방법시배육항병품충,은성항병기인xa5시중요적수도백협고병항성기인。통과구건현성급은성 xa5기인적원핵표체재체(PGEX-4T-1-Xa5화PGEX-4T-1-xa5),전화대장간균BL21,경0.2 mmol/L IPTG 28℃유도4 h,표체현성Xa5화은성xa5단백；결합western-blotting분석,결과표명표체적단백시대유GST표첨적융합단백,최후이용곡광감태친화층석주장단백순화,위후기연구xa5기인적공능제공기출。
Rice is an economically important crop, the output of rice is closely related to the factors such as plant diseases and insect pests. Bacterial blight ( BB) caused by Xanthomonas oryzae pv. Oryzae (Xoo) is one of three most destructive diseases in rice which cased output reduce. The cultivation of varieties with high resistance is considered as the most effective and economical option to control this disease. xa5 recessive resistance gene is an important rice bacterial leaf blight resistance genes. In this study, we construct prokaryotic expression vector (PGEX-4T-1-Xa5 and PGEX-4T-1-xa5), and translate it into BL21, then 0.2 mmol/L IPTG, 28℃, 4 h induced dominant Xa5 and recessive xa5 protein expression. Then in combination with western-blotting analysis, show that the expression of protein is a GST fusion protein and purification of the protein, These results laid a foundation for elucidating characteristic and function of xa5.